Dynamics of the cytosolic chelatable iron pool of K562 cells

FEBS Letters
W BreuerZ I Cabantchik

Abstract

The labile iron pool of cells (LIP) constitutes the primary source of metabolic and catalytically reactive iron in the cytosol. We studied LIP homeostasis in K562 cells using the fluorescent metal-sensitive probe calcein. Following brief exposure to iron(II) salts or to oxidative or reductive stress, LIP rose by up to 120% relative to the normal level of 350nM. However, the rate of recovery to normal LIP level differed markedly with each treatment (respective t1/2s of 27, 65-88 and < or = 17 min). We show that the capacity of K562 cells to adjust LIP levels is highly dependent on the origin of the LIP increase and on the pre-existing cellular iron status.

References

Jan 1, 1992·Critical Reviews in Toxicology·T P Ryan, S D Aust
Mar 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·E BakerP Ponka
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A ConstableM W Hentze
Jan 1, 1990·Methods in Enzymology·B Halliwell, J M Gutteridge
Jan 1, 1990·Methods in Enzymology·M S Sussman, G B Bulkley
Jan 1, 1988·Critical Reviews in Clinical Laboratory Sciences·C Hershko, D J Weatherall
Jan 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H KondoP Aisen
May 1, 1988·Toxicology and Applied Pharmacology·D W ReifS D Aust
Oct 1, 1985·European Journal of Biochemistry·F FunkW Schneider
Oct 13, 1995·The Journal of Biological Chemistry·W BreuerZ I Cabantchik
Jun 1, 1995·The American Journal of Physiology·W BreuerI Z Cabantchik
Feb 10, 1995·The Journal of Biological Chemistry·I TothK R Bridges
Apr 1, 1994·Free Radical Biology & Medicine·F MagniN Paolocci
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J BallaG M Vercellotti
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M ChevionN Kitrossky

❮ Previous
Next ❯

Citations

Mar 23, 2002·Free Radical Biology & Medicine·Mario Comporti
Oct 11, 2002·Free Radical Biology & Medicine·Or Kakhlon, Z Ioav Cabantchik
Jun 20, 1998·British Journal of Haematology·C HershkoG Link
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·C PourzandR M Tyrrell
Oct 18, 2005·The Journal of Biological Chemistry·Ambreena SiddiqRajiv R Ratan
May 30, 2002·Biological Chemistry·Frank PetratUrsula Rauen
Sep 3, 2013·Antioxidants & Redox Signaling·Claudia N Paiva, Marcelo T Bozza
Apr 24, 2002·Analytical Biochemistry·Breno P EspósitoZ Ioav Cabantchik
Sep 18, 2004·The Journal of Investigative Dermatology·Julia Li ZhongCharareh Pourzand
May 20, 2006·The Journal of Investigative Dermatology·Anthie YiakouvakiCharareh Pourzand
May 28, 2011·Free Radical Biology & Medicine·Andzelika BorkowskaJedrzej Antosiewicz
Apr 22, 2004·The Journal of Pharmacy and Pharmacology·Wei LuoZu D Liu
May 20, 2004·Journal of Inorganic Biochemistry·Kenneth AbreoSushil Jain
Nov 20, 2014·Chemical Society Reviews·Hao ZhuXiaojun Peng
Mar 3, 2004·Journal of Biochemical and Molecular Toxicology·W IbrahimC K Chow
Apr 18, 2018·The Journal of Biological Chemistry·Fernando Cruvinel DamascenoJosé Carlos Toledo
Dec 13, 2005·Annals of the New York Academy of Sciences·Chaim HershkoZ Ioav Cabantchik
Oct 12, 2011·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Asma ArounCharareh Pourzand
May 30, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tian TangCanghai Li
Dec 22, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Apriliana E R KartikasariJoannes J M Marx
May 1, 1999·The Journal of Biological Chemistry·F ThomasJ P Laulhère
Aug 8, 1998·The American Journal of Physiology·A Stäubli, U A Boelsterli
Jul 21, 1998·Annals of the New York Academy of Sciences·C HershkoI Cabantchik

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.