Dynamin II associates with Grb2 SH3 domain in Ras transformed NIH3T3 cells

Biochemical and Biophysical Research Communications
S Y YoonM Y Han

Abstract

Grb2, a linker protein containing two SH3 domains and one SH2 domain, is known as an essential element of the Ras pathway in multiple systems. One of the functions of Grb2 is to link tyrosine-phosphorylated receptors to downstream effector proteins via the SH2 and SH3 domain bindings. To identify Grb2-associated proteins in Ras transformed NIH3T3 cells, we performed coprecipitation experiments using recombinant GST-Grb2 fusion proteins and found a remarkably strong band of 100 kDa. With N-terminal amino acid sequencing, we identified the protein of 100 kDa as dynamin II. Dynamin II was also observed in the coprecipitates with the GST fusion protein of N-SH3 or C-SH3 domain of Grb2 but not in that of Grb2 SH2 domain. The SH3-mediated association of Grb2 with dynamin II was confirmed by competitive binding experiments with oligopeptides whose sequence corresponded to that of SH2 or SH3 binding motif. The dynamin II coprecipitation was completely abrogated by the addition of the oligopeptide of SH3 binding motif, but addition of SH2 binding motif had no effect. In conclusion, these results suggest that dynamin II may be largely expressed and closely associated with Grb2-mediated signaling in Ras transformed cells.

References

Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J S HerskovitsR B Vallee
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·S KharbandaD Kufe
Aug 4, 1995·Biochemical and Biophysical Research Communications·P CrespoJ S Gutkind
Mar 12, 1993·Cell·Z SongyangR J Lechleider
Mar 9, 1995·Nature·R B Kelly
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·T A CookM A McNiven
Jul 16, 1993·Cell·D Bar-SagiJ Schlessinger
May 1, 1996·The Journal of Cell Biology·J R Henley, M A McNiven
Jun 14, 1996·Biochemical and Biophysical Research Communications·O MaierP Westermann

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Citations

Oct 14, 2000·Annual Review of Cell and Developmental Biology·J E Hinshaw
Apr 27, 2001·Biochemical and Biophysical Research Communications·M J JeongM Y Han
Feb 4, 2010·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Anne-Cécile DurieuxMarc Bitoun
Nov 17, 2009·Immunological Reviews·Konstantin NeumannJürgen Wienands
Feb 8, 2013·PloS One·Shameka J ShelbyDebra A Thompson
Dec 18, 2001·Journal of Cellular Biochemistry·S Y YoonM Y Han
Apr 23, 2008·Current Protocols in Protein Science·Martin R Schiller
Aug 9, 2006·Acta Biochimica Et Biophysica Sinica·Soon-Jeong JeongMoon-Jin Jeong
Aug 28, 1997·Biochemical and Biophysical Research Communications·F Ramos-MoralesM Tortolero
Aug 8, 2021·International Journal of Molecular Sciences·Nadezhda BarvitenkoAntonella Pantaleo

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