PMID: 11341978May 9, 2001Paper

Dynamin inhibits phosphatidylinositol 3-kinase in hematopoietic cells

Biochimica Et Biophysica Acta
D Harrison-FindikP J Robinson

Abstract

Phosphatidylinositol 3-kinase (PI 3-kinase) plays a role in late stages of endocytosis as well as in cellular proliferation and transformation. The SH3 domain of its regulatory p85 subunit stimulates the GTPase activity of dynamin in vitro. Dynamin is a GTPase enzyme required for endocytosis of activated growth factor receptors. An interaction between these proteins has not been demonstrated in vivo. Here, we report that dynamin associates with PI 3-kinase in hematopoietic cells. We detected both p85 and PI 3-kinase activity in dynamin immune complexes from IL-3-dependent BaF3 cells. However, this association was significantly reduced in BaF3 cells transformed with the BCR/abl oncogene. After transformation only a 4-fold increase in PI 3-kinase activity was detected in dynamin immune complexes, whereas grb2 associated activity was elevated 20-fold. Furthermore, dynamin inhibited the activity of both purified recombinant and immunoprecipitated PI 3-kinase. In BaF3 cells expressing a temperature-sensitive mutant of BCR/abl, a significant decrease in p85 and dynamin association was observed 4 h after the induction of BCR/abl activity. In contrast, in IL-3-stimulated parental BaF3 cells, this association was increased. Our results ...Continue Reading

References

May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·N RosenbergC D Scher
Sep 1, 1978·The Journal of Experimental Medicine·R RisserW P Rowe
Feb 1, 1991·Molecular and Cellular Biology·L VarticovskiL C Cantley
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·N Bonnefoy-BérardA Altman
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J S HerskovitsR B Vallee
Apr 12, 1994·Biochimica Et Biophysica Acta·L VarticovskiM Susa
Aug 1, 1994·Trends in Neurosciences·P J RobinsonT C Südhof
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·S KharbandaD Kufe
Mar 1, 1994·Journal of Biomolecular NMR·J SchleucherC Griesinger
Aug 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R Kapeller, L C Cantley
Aug 1, 1993·The Journal of Cell Biology·A M van der BliekS L Schmid
Mar 1, 1993·Molecular and Cellular Biology·C L CarpenterL C Cantley
Sep 1, 1996·Journal of Neurochemistry·T CookR Urrutia
Apr 1, 1996·Current Opinion in Cell Biology·C L Carpenter, L C Cantley
Aug 1, 1997·Current Biology : CB·P WiggeH T McMahon
Nov 5, 1997·Molecular and Cellular Endocrinology·J P LiuS Kawashima
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·H C LinA G Gilman
Oct 6, 1998·Annual Review of Biochemistry·D A FrumanL C Cantley
Apr 10, 1999·The Journal of Biological Chemistry·J L DeGraffM J Orsini
Aug 24, 1999·The Journal of Biological Chemistry·J L Whistler, M von Zastrow
Mar 1, 1996·Trends in Cell Biology·P R ShepherdH W Davidson

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.