Dynein-deficient flagella respond to increased viscosity with contrasting changes in power and recovery strokes

Cytoskeleton
Kate S WilsonP V Bayly

Abstract

Changes in the flagellar waveform in response to increased viscosity were investigated in uniflagellate mutants of Chlamydomonas reinhardtii. We hypothesized that the waveforms of mutants lacking different dynein arms would change in different ways as viscosity was increased, and that these variations would illuminate the feedback pathways from force to dynein activity. Previous studies have investigated the effects of viscosity on cell body motion, propulsive force, and power in different mutants, but the effect on waveform has not yet been fully characterized. Beat frequency decreases with viscosity in wild-type uniflagellate (uni1) cells, and outer dynein arm deficient (oda2) mutants. In contrast, the inner dynein arm mutant ida1 (lacking I1/f) maintains beat frequency at high viscosity but alters its flagellar waveform more than either wild-type or oda2. The ida1 waveform is narrower than wild-type, primarily due to an abbreviated recovery stroke; this difference is amplified at high viscosity. The oda2 mutant in contrast, maintains a consistent waveform at high and low viscosity with a slightly longer power stroke than wild-type. Analysis of the delays and shear displacements between bends suggest that direct force feedbac...Continue Reading

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Citations

Nov 1, 2016·Journal of the Royal Society, Interface·P V Bayly, S K Dutcher
Jun 1, 2018·American Journal of Respiratory Cell and Molecular Biology·Alina OlteanSteven L Brody
Oct 4, 2018·Molecular Biology of the Cell·David S BoothNicole King
Jun 28, 2019·Journal of Cell Science·Anthony D JunkerChad G Pearson

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