Dynein light chain 1 functions in somatic cyst cells regulate spermatogonial divisions in Drosophila.

Scientific Reports
Prakash JotiKrishanu Ray

Abstract

Stem cell progeny often undergo transit amplifying divisions before differentiation. In Drosophila, a spermatogonial precursor divides four times within an enclosure formed by two somatic-origin cyst cells, before differentiating into spermatocytes. Although germline and cyst cell-intrinsic factors are known to regulate these divisions, the mechanistic details are unclear. Here, we show that loss of dynein-light-chain-1 (DDLC1/LC8) in the cyst cells eliminates bag-of-marbles (bam) expression in spermatogonia, causing gonial cell hyperplasia in Drosophila testis. The phenotype is dominantly enhanced by Dhc64C (cytoplasmic Dynein) and didum (Myosin V) loss-of-function alleles. Loss of DDLC1 or Myosin V in the cyst cells also affects their differentiation. Furthermore, cyst cell-specific loss of ddlc1 disrupts Armadillo, DE-cadherin and Integrin-βPS localizations in the cyst. Together, these results suggest that Dynein and Myosin V activities, and independent DDLC1 functions in the cyst cells organize the somatic microenvironment that regulates spermatogonial proliferation and differentiation.

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Citations

Oct 23, 2012·Spermatogenesis·Richard Zoller, Cordula Schulz
Mar 13, 2012·Medical Hypotheses·María-Angeles AllerJaime Arias
Apr 21, 2016·Molecular & Cellular Proteomics : MCP·Sira DefausRicardo Gutiérrez-Gallego
Oct 15, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Adrien BosseboeufPascal Sourdaine
Jun 2, 2017·Journal of Biosciences·Samir Gupta, Krishanu Ray
Aug 23, 2018·Histochemistry and Cell Biology·Tomohide UnoAkira Mizoguchi
Nov 17, 2020·Oxidative Medicine and Cellular Longevity·Wanyin ChenJie Fang
Jun 6, 2019·Development·John E La MarcaW Gregory Somers

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Methods Mentioned

BETA
dissection
nucleotide exchange
transgenic
Fluorescence

Software Mentioned

Adobe Photoshop
Image
FV10
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