PMID: 6112743Feb 1, 1981Paper

Dynorphin immunocytochemical localization in brain and peripheral nervous system: preliminary studies

Proceedings of the National Academy of Sciences of the United States of America
S J WatsonA Goldstein

Abstract

Using antisera specific for the opioid peptide dynorphin, we have carried out immunocytochemical studies of the distribution in rat brain and periphery. In the central nervous system, cells that stain positively for dynorphin are found in the supraoptic nucleus, with less-well-stained cells in the paraventricular nucleus of the hypothalamus. Few positive fibers were detected in brain, suggesting problems with fixation and preservation of antigenicity. In pituitary no staining was seen in the anterior and intermediate lobes but heavy staining was detected in the posterior lobe. In the guinea pig, adrenal chromaffin cells stained with dynorphin antisera. Staining of these cells could be blocked with excess of dynorphin-(1-13) or either enkephalin. Radioimmunoassays revealed a great excess of the enkephalins in the adrenal, suggesting cross competition between dynorphin antiserum and adrenal medullary enkephalin. Finally, the dynorphin antiserum stained a complex of fibers in guinea pig ileum. Staining of these fibers could be blocked by moderate amounts of enkephalin as well as by smaller amounts of dynorphin-(1-13). We conclude that in some places (brain and pituitary) dynorphin exists separately from leucine-enkephalin. In othe...Continue Reading

References

May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R SimantovS H Snyder
Jul 10, 1978·Life Sciences·H AkilJ D Barchas
Sep 21, 1978·Nature·S J WatsonJ D Barchas
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A GoldsteinL Hood
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S J WatsonC H Li
Jan 31, 1978·Cell and Tissue Research·E A ZimmermanD T Krieger
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Jul 7, 1980·Life Sciences·V E GhazarossianA Goldstein
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A Goldstein, V E Ghazarossian

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Citations

Oct 1, 1991·Journal of Clinical Pharmacology·R L Kohl, S MacDonald
Aug 1, 1986·Naunyn-Schmiedeberg's Archives of Pharmacology·R SchulzC Gramsch
Mar 1, 1985·Naunyn-Schmiedeberg's Archives of Pharmacology·W Knepel, M Schwaninger
Dec 16, 2011·Amino Acids·Jana Ninković, Sabita Roy
Feb 4, 1982·Brain Research·C GramschA Herz
Jan 22, 1982·European Journal of Pharmacology·T OkaM Wakimasu
Jul 9, 1982·European Journal of Pharmacology·J F Kachur, R J Miller
Jan 1, 1983·Neuropeptides·S Spampinato, A Goldstein
Mar 1, 1987·Regulatory Peptides·F Nyberg, I Nylander
Sep 1, 1993·Brain Research. Molecular Brain Research·R SpanglerM J Kreek
Nov 1, 1982·Peptides·H KhachaturianH Akil
Nov 1, 1982·Peptides·G A OlsonD H Coy
Jan 1, 1985·Peptides·H KhachaturianS J Watson
Jan 1, 1982·Progress in Neurobiology·R C Frederickson, L E Geary
Dec 1, 1993·Progress in Neurobiology·V ReghunandananP I Singh
Oct 20, 1980·Neuroscience Letters·B M CoxA Goldstein
Nov 30, 1982·Neuroscience Letters·S R VincentL Terenius
Sep 7, 1984·Neuroscience Letters·E NishimuraC H McIntosh
Apr 1, 1984·Neuroscience·S R VincentL Terenius
Jul 1, 1982·Brain Research Bulletin·H Khachaturian, S J Watson
Apr 1, 1992·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·K Unsicker, F Stögbauer
Aug 1, 1994·Journal of Chemical Neuroanatomy·M FodorM Palkovits
Nov 8, 2001·European Journal of Pharmacology·R Przewłocki, B Przewłocka
Dec 1, 1996·Progress in Neurobiology·M Simonato, P Romualdi

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