Dyrk kinases regulate phosphorylation of doublecortin, cytoskeletal organization, and neuronal morphology.

Cytoskeleton
Tatiana I SlepakJohn L Bixby

Abstract

In a neuronal overexpression screen focused on kinases and phosphatases, one "hit" was the dual specificity tyrosine phosphorylation-regulated kinase (Dyrk4), which increased the number of dendritic branches in hippocampal neurons. Overexpression of various Dyrk family members in primary neurons significantly changed neuronal morphology. Dyrk1A decreased axon growth, Dyrk3 and Dyrk4 increased dendritic branching, and Dyrk2 decreased both axon and dendrite growth and branching. Kinase-deficient mutants revealed that most of these effects depend on kinase activity. Because doublecortin (DCX), a microtubule-binding protein, regulates cytoskeletal dynamics and neuronal morphogenesis, we investigated the possibility that DCX is a target of Dyrks. We found that overexpression of Dyrk2 and Dyrk3, but not Dyrk1A or Dyrk4, can change DCX phosphorylation status. Mutation of a consensus phosphorylation site for Dyrk kinases at Serine 306 (Ser306) in DCX indicated that this is one target site for Dyrk2 and Dyrk3. Overexpression of Dyrk2 restored altered DCX distribution in the growth cones of dendrites and axons, and partially reversed the morphological effects of DCX overexpression; some of these effects were abrogated by mutation of Ser3...Continue Reading

References

Feb 5, 1999·Progress in Nucleic Acid Research and Molecular Biology·W Becker, H G Joost
May 1, 2002·The Journal of Biological Chemistry·Seunghwan LimEileen Friedman
Apr 15, 2003·Nature Structural Biology·Myung Hee KimZygmunt S Derewenda
Apr 26, 2003·Nature Structural Biology·Carolyn A MooresAnne Houdusse
Jun 12, 2003·Journal of Cell Science·Monica AlvarezSusana de la Luna
Oct 29, 2003·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Erik W DentKatherine Kalil
Feb 7, 2004·The EMBO Journal·Amos GdalyahuOrly Reiner
Apr 22, 2004·The Biochemical Journal·Mark E GrahamPeter L Jeffrey
May 1, 2004·Cell Cycle·Orly ReinerTamar Sapir
Jul 27, 2004·Protein Science : a Publication of the Protein Society·Natarajan Kannan, Andrew F Neuwald
Jan 19, 2007·Molecular Biology of the Cell·Mónica AlvarezSusana de la Luna
Aug 24, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ali ErtürkFrank Bradke
Dec 14, 2007·Developmental Neuroscience·Takeshi Kawauchi, Mikio Hoshino
Dec 14, 2007·Developmental Neuroscience·Dror CohenOrly Reiner
Mar 17, 2009·Nature Cell Biology·Subbareddy Maddika, Junjie Chen
Mar 25, 2009·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Corinna Darian-Smith
Apr 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Géraldine KerjanJoseph G Gleeson
Jun 25, 2009·Journal of Cell Science·Timothy M E ScalesPhillip R Gordon-Weeks
Sep 4, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Irina TintMark M Black
Dec 8, 2009·The Journal of Biological Chemistry·Naoe TairaKiyotsugu Yoshida
Feb 18, 2010·Molecular and Cellular Neurosciences·Murray G BlackmoreVance P Lemmon
Apr 2, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lynn C UsherJohn L Bixby
Jul 29, 2010·Molecular Systems Biology·William J BuchserVance P Lemmon
Sep 14, 2010·Current Opinion in Neurobiology·Erik W DentXindao Hu
Nov 5, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sergi ArandaSusana de la Luna
Dec 16, 2010·The FEBS Journal·Francisco J Tejedor, Barbara Hämmerle
Dec 16, 2010·Journal of Neuropathology and Experimental Neurology·Jerzy WegielCheng-Xin Gong

❮ Previous
Next ❯

Citations

Jul 2, 2015·Journal of Molecular Neuroscience : MN·Runchuan YanShanting Zhao
Jun 18, 2016·Small GTPases·Susana A Gonçalves, Tiago Fleming Outeiro
Sep 11, 2019·FEBS Letters·Saishu Yoshida, Kiyotsugu Yoshida
Feb 25, 2017·Frontiers in Molecular Neuroscience·Morten T VenøJørgen Kjems
May 29, 2020·Cellular and Molecular Life Sciences : CMLS·Alejandro Correa-SáezMarco A Calzado
Nov 7, 2019·Journal of Alzheimer's Disease : JAD·Karol DowjatYu-Wen Hwang
Apr 4, 2021·International Journal of Molecular Sciences·Kyeongmin KimBuHyun Youn
Jul 3, 2021·International Journal of Molecular Sciences·Mattias F Lindberg, Laurent Meijer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.