DYS STR analysis with epithelial cells in a rape case

Forensic Science International : Synergy
A BetzW Pflug

Abstract

We report here the application of Y-chromosomal DNA analysis in a rape case, which occurred in Stuttgart, Germany. Microscopic examination of the victim's vaginal swabs and her underwear showed no sperm cells. DNA was extracted from vaginal and epithelial cells and analysed with the autosomal systems SE33, THO1 (singleplex) and with the multiplex Profiler Plus (Applied Biosystems, Foster City, USA). The results of these autosomal STR analysis gave no hint at a mixed sample and failed to identify a male profile. DYS STR analysis with the systems DYS391, DYS392, DYS393, DYS19 and DYS389 I/II showed the same characteristic features as the suspect. We used this incomplete haplotype to search in the Y-STR Haplotype Reference Database via Internet. In a Caucasian population sample of 3589 minimal haplotypes we found 71 matches. The suspect confessed the crime and was finally condemned to 4 years imprisonment.

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Citations

May 21, 2009·Forensic Science, Medicine, and Pathology·Lutz Roewer
Mar 26, 2004·Forensic Science International : Synergy·Aydin RustamovIşik Bökesoy
Dec 13, 2002·Forensic Science International : Synergy·Alan J ReddMichael F Hammer
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Dec 3, 2014·Forensic Science International. Genetics·Andrew McDonaldPaula O'Rourke
Jul 6, 2014·Forensic Science International. Genetics·Germana Emanuela De Queiroz RêgoNaila Francis Paulo De Oliveira
May 3, 2015·Forensic Science International. Genetics·Toni M Diegoli
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Apr 7, 2012·Genetics and Molecular Biology·Pablo Abdon da Costa FrancezSidney Emanuel Batista Dos Santos
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Aug 30, 2003·Analytical Chemistry·T A BrettellR Saferstein

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