Dysbiosis of Intestinal Microbiota and Decreased Antimicrobial Peptide Level in Paneth Cells during Hypertriglyceridemia-Related Acute Necrotizing Pancreatitis in Rats

Frontiers in Microbiology
Chunlan HuangYue Zeng

Abstract

Hypertriglyceridemia (HTG) aggravates the course of acute pancreatitis (AP). Intestinal barrier dysfunction is implicated in the pathogenesis of AP during which dysbiosis of intestinal microbiota contributes to the dysfunction in intestinal barrier. However, few studies focus on the changes in intestine during HTG-related acute necrotizing pancreatitis (ANP). Here, we investigated the changes in intestinal microbiota and Paneth cell antimicrobial peptides (AMPs) in HTG-related ANP (HANP) in rats. Rats fed a high-fat diet to induce HTG and ANP was induced by retrograde injection of 3.5% sodium taurocholate into biliopancreatic duct. Rats were sacrificed at 24 and 48 h, respectively. Pancreatic and ileal injuries were evaluated by histological scores. Intestinal barrier function was assessed by plasma diamine oxidase activity and D-lactate level. Systemic and intestinal inflammation was evaluated by tumor necrosis factor alpha (TNFα), interleukin (IL)-1β, and IL-17A expression. 16S rRNA high throughput sequencing was used to investigate changes in intestinal microbiota diversity and structure. AMPs (α-defensin5 and lysozyme) expression was measured by real-time polymerase chain reaction (PCR) and immunofluorescence. The results s...Continue Reading

References

Jan 1, 1992·Annals of Surgery·J SchmidtA L Warshaw
Feb 8, 2008·Journal of Zhejiang University. Science. B·Xi-ping ZhangHan-qin Chen
Mar 12, 2008·HPB : the Official Journal of the International Hepato Pancreato Biliary Association·R S Flint, J A Windsor
May 29, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Claire Barbier de La SerreHelen E Raybould
Jul 29, 2010·Physiological Reviews·Inna SekirovB Brett Finlay
Jan 11, 2011·Current Opinion in Microbiology·Ivaylo I Ivanov, Dan R Littman
Mar 11, 2011·Inflammatory Bowel Diseases·Nabeetha A NagalingamVincent B Young
Nov 19, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Zora TeltschikJan Wehkamp
Feb 1, 2012·The Journal of Experimental Medicine·Michael H ShawGabriel Núñez
Mar 6, 2012·Hepato-gastroenterology·Yue ZengJingjing Ma
Sep 14, 2012·Journal of Clinical Gastroenterology·Gabriele CapursoGianfranco Delle Fave
Feb 13, 2013·Annual Review of Physiology·Hans C Clevers, Charles L Bevins
Oct 4, 2013·Nature·Timon E AdolphRichard S Blumberg
Sep 6, 2014·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Alice SabatinoEnrico Fiaccadori
Oct 2, 2014·European Journal of Internal Medicine·Pedro ValdivielsoNils Ewald
Oct 2, 2015·The Journal of Nutritional Biochemistry·Jessie BaldwinMichael K McIntosh
Feb 14, 2016·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·JunYuan ZhengYue Zeng
May 21, 2016·Bioscience of Microbiota, Food and Health·Kiminori NakamuraTokiyoshi Ayabe
Nov 12, 2016·World Journal of Gastroenterology : WJG·Mei Zhang, Xiao-Jiao Yang

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Citations

Sep 19, 2017·Probiotics and Antimicrobial Proteins·Banafsheh Douzandeh-Mobarrez, Ashraf Kariminik
May 30, 2019·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Luqiao HuangChunbao Guo
Dec 19, 2019·Critical Reviews in Microbiology·Jinbo LiuXianming Xia
Jun 8, 2019·International Journal of Molecular Medicine·Nosheen MushtaqJiru Xu
Dec 7, 2018·Journal of Gastroenterology·Yin ZhuNonghua Lu
Mar 16, 2019·Frontiers in Microbiology·Lu XiaoJie Chen
Mar 27, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Qihui LuoZhengli Chen
Aug 23, 2019·Annual Review of Nutrition·Timon E AdolphHerbert Tilg
May 11, 2018·Journal of Gastroenterology and Hepatology·Yan-Jie ChenXi-Zhong Shen
May 19, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Qiuhua LianHongwen Sun
Mar 10, 2020·Canadian Journal of Physiology and Pharmacology·Yuanjiao LiangTao Shen
May 4, 2021·Digestive Diseases and Sciences·Lu WangDong Wu
Jul 3, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ying ZhuYue Zeng
Jul 15, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Yan-Hua ZhengTong Yang
Jul 7, 2021·Journal of the Science of Food and Agriculture·Chenyu XueAnshan Shan

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Datasets Mentioned

BETA
SRP081324

Methods Mentioned

BETA
protein assay
enzyme-linked immunosorbent assay
PCR
electrophoresis
ELISA
PCA

Software Mentioned

UPARSE
UCHIME
Mothur
RDP Classifier
R
SPSS
gplot
Trimmomatic
FLASH

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