Dysbiosis of the fecal microbiota in the TNBS-induced Crohn's disease mouse model

Applied Microbiology and Biotechnology
Qing HeLiang Xiao

Abstract

Crohn's disease (CD) is characterized by chronic transmural inflammation. The symptom of the mice model induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) is closed to human under CD condition, so this kind of animal is widely used in the related researches. Although the dysbiosis of the fecal microbiota has been proved to play an important role in the patients with CD, the composition of the gastrointestinal microbiota in the mouse model under disease condition is still unclear. In the current study, male 7-week BALB/c mice were anesthetized and intrarectal administrated by ethanol (ET group), TNBS in ethanol (TN group), and phosphate buffered saline (PBS) (CK group) as control. The symptoms of individuals under the CD condition were observed, and the changes of the bacterial taxonomic structure and functional composition were revealed by next-generation sequencing (NGS) 16S sequencing. The BALB/c mice in TN group demonstrated CD-like symptoms and the damages in the intestinal tract. The NGS 16S results exhibited that the diversity and microbial composition under CD condition are significantly different with those in ET group. The KEGG Orthology (KO) profile were generated from PICRUSt, and function modules such as methanoge...Continue Reading

References

Nov 1, 1995·The Journal of Experimental Medicine·M F NeurathW Strober
May 24, 2003·Trends in Molecular Medicine·Theresa T PizarroFabio Cominelli
Dec 20, 2003·Gut·C P TamboliJ F Colombel
Jul 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ruth E LeyJeffrey I Gordon
Jul 6, 2006·Applied and Environmental Microbiology·T Z DeSantisG L Andersen
Oct 3, 2006·Inflammatory Bowel Diseases·Anje A te VeldeDaniel W Hommes
Mar 3, 2007·The Journal of Clinical Investigation·Warren StroberPeter Mannon
Apr 11, 2008·The ISME Journal·Johan DicksvedJanet K Jansson
Oct 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Harry SokolPhilippe Langella
Jan 20, 2010·Journal of Ethnopharmacology·Maria Silene da SilvaCatalina Alarcón de La Lastra
Mar 3, 2010·Annual Review of Immunology·Arthur KaserRichard S Blumberg
Jun 17, 2011·Nature·Kevin J Maloy, Fiona Powrie
Mar 28, 2013·The Journal of Clinical Endocrinology and Metabolism·R MathurM Pimentel
Aug 27, 2013·Nature Biotechnology·Morgan G I LangilleCurtis Huttenhower
Aug 30, 2013·Nature·Emmanuelle Le ChatelierOluf Pedersen
Jul 16, 2014·Journal of the American Chemical Society·Alexey KulikovskySvetlana Dubiley
Aug 12, 2014·Cancer Prevention Research·Joseph P ZackularPatrick D Schloss
Sep 2, 2014·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Puneet Kaur RandhawaAmteshwar Singh Jaggi
Jan 7, 2015·World Journal of Gastroenterology : WJG·Emilien LoeuillardRachel Marion-Letellier
Jul 25, 2015·Science·Mohamed S Donia, Michael A Fischbach
Oct 16, 2015·Scientific Reports·Yingying ZhuGuanghong Zhou

❮ Previous
Next ❯

Citations

Apr 19, 2018·PLoS Computational Biology·Geoffrey D HanniganPatrick D Schloss
Nov 26, 2018·Journal of Cellular and Molecular Medicine·Yanrong LiuCheng Yang
Jan 22, 2019·Nature Medicine·Bahtiyar YilmazAndrew J Macpherson
Feb 27, 2018·Gastroenterology Report·Shaomin ZouMong-Hong Lee
Apr 18, 2019·Applied Microbiology and Biotechnology·Sheng LiuXiuqing Zhang
May 18, 2020·Poultry Science·Annatachja De GrandeRichard Ducatelle
Mar 8, 2020·The Journal of Nutritional Biochemistry·Ahmad Ud DinGuixue Wang
Jun 30, 2021·Nature Medicine·Benjamin M ScottFrancisco J Quintana
Oct 30, 2021·Biopreservation and Biobanking·Mu ChenBaolin Liu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.