Abstract
We studied the ability of monocytes to metabolize [3H]arachidonic acid (AA) provided exogenously by activated T cells, and the extent to which dexamethasone suppressed eicosanoid production by normal and atopic cells. [3H]AA metabolites were identified using a reverse-phase high pressure liquid chromatography system (HPLC). Unstimulated and PHA-stimulated T cells from normal and atopic subjects exhibited a similar uptake and time-dependent release of radiolabel, 90% of which was identified as free AA. The addition of autologous normal and atopic monocytes to these cultures enhanced the release of radiolabel, even in the absence of stimulation with mitogen. Atopic T cell/monocyte cultures released significantly (P = 0.046) more radiolabel than normal cells when stimulated with PHA. Furthermore, the monocytes from both normal and atopic subjects metabolized T cell derived [3H]AA into cyclo-oxygenase (CO) and lipoxygenase (LO) products. Under unstimulated conditions, atopic cells produced significantly (P = 0.04) less CO products than normal cells. In contrast, under PHA and calcium ionophore-stimulated conditions, the atopic cells produced significantly (P = 0.048) more prostaglandins than normal donor cells. Furthermore, althoug...Continue Reading
References
May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P Borgeat, B Samuelsson
Jun 1, 1979·The Journal of Experimental Medicine·C W ParkerM G Huber
Jan 1, 1988·The Journal of Allergy and Clinical Immunology·B StyrtM S Klempner
Sep 9, 1985·Life Sciences·H MitaT Shida
Mar 1, 1986·The Journal of Allergy and Clinical Immunology·S R WangB N Chiang
Jan 1, 1986·Lipids·R E RocklinD Horrobin
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J Carlstedt-DukeB Strandvik
Mar 1, 1988·Clinical Immunology and Immunopathology·C AuderaD Deykin
May 1, 1985·Biochemical Pharmacology·J ChangA J Lewis
Sep 1, 1984·The Journal of Allergy and Clinical Immunology·M E Goldyne
Nov 25, 1983·Journal of Immunological Methods·S P PetersL M Lichtenstein
Apr 1, 1984·The Journal of Clinical Investigation·R A Lewis, K F Austen
Jan 1, 1984·Journal of Leukocyte Biology·R J Bonney, J L Humes
Jun 1, 1984·The British Journal of Dermatology·M S MankuJ L Burton
Mar 1, 1984·The Journal of Allergy and Clinical Immunology·M Kalinen
Aug 1, 1983·The Journal of Experimental Medicine·N A PawlowskiW A Scott
Sep 1, 1984·The Journal of Allergy and Clinical Immunology·N A PawlowskiW A Scott
Aug 1, 1984·Agents and Actions·D M Weir
Oct 1, 1983·The Journal of Allergy and Clinical Immunology·S M MatloffR E Rocklin
Feb 1, 1981·Cellular Immunology·M G GoodmanW O Weigle
Jul 1, 1981·The Medical Clinics of North America·E J Goetzl
Apr 15, 1982·The Biochemical Journal·R F Irvine
Nov 1, 1982·Prostaglandins·M E Goldyne, J D Stobo
Aug 1, 1980·The Journal of Experimental Medicine·W A ScottZ A Cohn
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Oct 1, 1985·Immunology Today·M Rola-Pleszczynski
Citations
Apr 1, 1990·Inflammation·L BorishL J Rosenwasser
Nov 29, 2001·Lipids·K Duchén, B Björkstén
Oct 1, 1991·Allergy·B MelnikT Tschung
Nov 1, 1991·Journal of the American Academy of Dermatology·B Melnik, G Plewig
Feb 1, 1991·Prostaglandins, Leukotrienes, and Essential Fatty Acids·B C MelnikT Tschung
Jun 1, 1993·Prostaglandins, Leukotrienes, and Essential Fatty Acids·K Ikai, S Imamura
Nov 19, 1997·The Journal of International Medical Research·M AndreassiP Amerio