Dysregulation of arcuate nucleus preproneuropeptide Y mRNA in diet-induced obese rats

The American Journal of Physiology
B E Levin, Ambrose A Dunn-Meynell

Abstract

Neuropeptide Y (NPY) neurons in the hypothalamic arcuate nucleus (ARC) produce metabolic and physiological effects that promote the development and maintenance of obesity. In turn, NPY metabolism in these neurons is inhibited by dopamine release. In this study, ARC prepro-NPY mRNA and ARC/median eminence (ME) dopamine turnover were assessed in chow-fed male Sprague-Dawley rats prone to develop diet-induced obesity (DIO) or to be diet resistant (DR) when fed a high-energy (HE) diet. By in situ hybridization, DIO-prone rats had 39% more ARC NPY mRNA expression than DR-prone rats under chow-fed conditions. DIO-prone rat ARC/ME dopamine levels were 14% higher, but dopamine half-life was 176% longer and turnover was 59% less than DR-prone rats. Neither a 48-h fast nor 50% energy intake restriction for 5 days affected the already increased ARC NPY mRNA levels in DIO-prone rats. Both manipulations increased NPY expression to the level of DIO-prone rats in DR-prone rats by 23 and 35%, respectively. Finally, when fed HE diet for 2 wk, neither DIO- nor DR-prone rats altered their ARC NPY expression despite the development of obesity and hyperinsulinemia in DIO rats. Thus DIO-prone rats overexpress and fail to regulate ARC NPY mRNA to ene...Continue Reading

Citations

Oct 12, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Nancy C Tkacs, Barry E Levin
May 10, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Barry E LevinAmbrose A Dunn-Meynell
Mar 17, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jieyun YinJiande D Z Chen
Oct 28, 2010·Nutrition Research Reviews·Niloofar Hariri, Louise Thibault
Sep 10, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Belinda H McCullyBeth A Habecker
Jun 21, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Chantal MichelAmbrose A Dunn-Meynell
Sep 14, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Barry E Levin, Ambrose A Dunn-Meynell
Jan 13, 2006·American Journal of Physiology. Endocrinology and Metabolism·S F LeibowitzP P Pamy
Jul 22, 2006·Cellular and Molecular Neurobiology·Jia-Xu ChenZhu-Feng Wang
Jun 6, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Dalila Azzout-MarnichePatrick C Even
Jun 27, 2020·International Journal of Molecular Sciences·Shih-Yi LinChun-Jung Chen
Dec 18, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Barry E Levin, Ambrose A Dunn-Meynell
Sep 27, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·B E LevinR Servatius
Oct 12, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Barry E Levin, Ambrose A Dunn-Meynell
Nov 18, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·B E Levin, A A Dunn-Meynell
Nov 9, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Christa M PattersonBarry E Levin
Dec 26, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Barry E Levin, Ambrose A Dunn-Meynell
Jul 17, 2003·American Journal of Physiology. Endocrinology and Metabolism·Barry E LevinDavid E Cummings
Sep 23, 2000·American Journal of Physiology. Endocrinology and Metabolism·M ZiotopoulouJ S Flier
May 31, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Matthew R Ricci, Barry E Levin

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