Dysregulation of both miR-140-3p and miR-140-5p in synovial fluid correlate with osteoarthritis severity

Bone & Joint Research
C-M YinX-H Pan

Abstract

This study looked to analyse the expression levels of microRNA-140-3p and microRNA-140-5p in synovial fluid, and their correlations to the severity of disease regarding knee osteoarthritis (OA). Knee joint synovial fluid samples were collected from 45 patients with OA of the knee (15 mild, 15 moderate and 15 severe), ten healthy volunteers, ten patients with gouty arthritis, and ten with rheumatoid arthritis. The Kellgren-Lawrence grading (KLG) was used to assess the radiological severity of knee OA, and the patients were stratified into mild (KLG < 2), moderate (KLG = 2), and severe (KLG > 2). The expression of miR-140-3p and miR-140-5p of individual samples was measured by SYBR Green quantitative polymerase chain reaction (PCR) analysis. The expression of miR-140-3p and miR-140-5p was normalised to U6 internal control using the 2(-△△CT) method. All data were processed using SPSS software. Expression of both miR-140-3p and miR-140-5p was downregulated in OA synovial fluid, showing a statistical difference between the OA and non-OA group, and increased OA severity was associated with a decreased expression of miR-140-3p or miR-140-5p. The Spearman rank correlation analysis suggested that the expression of miR-140-3p or miR-140-...Continue Reading

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Citations

Feb 27, 2020·Biological & Pharmaceutical Bulletin·Tiantian RenLixin Huang
Jan 11, 2021·BMC Veterinary Research·Catarina CastanheiraMandy J Peffers
Oct 27, 2020·Bone & Joint Research·Chethan JayadevAndrew Price
May 1, 2021·International Journal of Molecular Sciences·Virginia Veronica ViscontiAnnalisa Botta
Jun 8, 2021·Chinese Journal of Natural Medicines·Jiu-Wang YuLu Wang
Jun 23, 2021·Journal of Orthopaedic Surgery and Research·Zhihuan LuoXiaguang Chen
Feb 2, 2022·Bone & Joint Research·Zhaxi LuobuLuosong Qunpei

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BETA
PCR

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SPSS

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