Dysregulation of CD69 by overexpression of microRNA‑367‑3p associated with post‑myocardial infarction cardiac fibrosis

Molecular Medicine Reports
Haiyan HuJingfang Zhang

Abstract

Cardiac fibrosis is characterized as net accumulation of ECM (extracellular matrix) proteins in the cardiac interstitium, which contributes to dysfunction of both systolic and diastolic. The present study aimed to identify the association between microRNA (miR)‑367‑3p and cluster of differentiation 69 (CD69), and their roles in regulating the development of cardiac fibrosis. Participants (n=34) were enrolled and diagnosed with cardiac fibrosis [fibrosis (+); n=16] or non‑fibrosis control [fibrosis (‑); n=18]. In‑silicon analysis and luciferase assay were used to identify CD69 as a target of miR‑367‑3p. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and western blot analysis were used to determine the expression level of miR‑367‑3p and CD69 mRNA and protein, in patient groups or cells transfected with miR‑367‑3p mimics or inhibitors. Cytokine assays were used to detect the level of interleukin (IL)‑17, tumor necrosis factor (TNF)‑α, interferon (IFN)‑γ and granulocyte macrophage colony‑stimulating factor. Flow cytometry was used to detect the T helper (Th)‑17 fraction of cells in different treatment groups. Analysis by RT‑qPCR indicated that the expression of miR‑367‑3p was decreased in the cardiac fibrosi...Continue Reading

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