Dysregulation of granulocyte, erythrocyte, and NK cell lineages in Fli-1 gene-targeted mice.

Blood
Masahiro MasuyaMakio Ogawa

Abstract

Targeted disruption of the Friend leukemia integration 1 (Fli-1) proto-oncogene results in severe dysmegakaryopoiesis and embryonic lethality. We used morula-stage aggregation as a strategy to further clarify the hematopoietic defects of the Fli-1 gene-targeted mice. Analyses of lineage expression of Fli-1(+/-) and Fli-1(-/-) cells in the peripheral blood and bone marrow of chimeric mice consistently demonstrated reduced numbers of neutrophilic granulocytes and monocytes and increased numbers of natural killer (NK) cells. Transplantation studies using sorted Fli-1 mutant cells produced similar findings. Clonal culture studies of bone marrow cells revealed increased numbers of granulocytic and early erythroid progenitors in the Fli-1(+/-) cells. The sorted Fli-1(-/-) bone marrow cells revealed specific down-regulation of CCAAT/enhancer binding protein-alpha (C/EBPalpha) and C/EBPepsilon, and the receptors for granulocyte colony-stimulating factor (G-CSF) and granulocyte-macrophage CSF (GM-CSF), consistent with their critical roles in granulopoiesis. Collectively, these observations suggest previously unknown physiologic roles for Fli-1 in granulocytic, erythroid, and NK cell proliferation and differentiation. Production of chime...Continue Reading

References

May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·L S ParkA Larsen
Jul 1, 1994·Journal of Cellular Biochemistry·R FelixH Fleisch
Jan 1, 1993·Molecular and Cellular Biology·V LemarchandelP H Roméo
Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y FujiwaraS H Orkin
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·D E ZhangD G Tenen
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·R YamanakaK G Xanthopoulos
May 28, 1998·The Journal of Biological Chemistry·A G RosmarinC P Simkevich
Feb 15, 2001·Oncogene·V I Sementchenko, D K Watson
Aug 16, 2001·International Journal of Hematology·Hiroshi KawadaMakio Ogawa
Mar 13, 2002·The Journal of Biological Chemistry·Athanasia D PanopoulosStephanie S Watowich
Mar 23, 2004·Journal of Cellular Biochemistry·Tien HsuDennis K Watson

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Citations

Nov 13, 2008·Molecular and Cellular Biology·Yoshihide AsanoMaria Trojanowska
Feb 24, 2007·Vascular Health and Risk Management·Mitsuko FuruyaHiroshi Ishikura
Dec 30, 2015·Current Rheumatology Reports·Kazuyuki Tsujino, Dean Sheppard
Jan 20, 2016·The Journal of Dermatology·Yoshihide Asano
Jul 29, 2010·Journal of Dermatological Science·Yoshihide AsanoMaria Trojanowska
Aug 25, 2010·Clinical and Experimental Immunology·J MatheniaX K Zhang
Mar 17, 2010·The American Journal of Pathology·Yoshihide AsanoMaria Trojanowska
Jul 4, 2007·Molecular Immunology·Tamara K NowlingGary S Gilkeson
Nov 3, 2012·PloS One·Elisenda Alari-PahissaPilar Lauzurica
Jul 9, 2016·Arteriosclerosis, Thrombosis, and Vascular Biology·Yanfang Peipei ZhuCatherine C Hedrick
Sep 9, 2005·The Journal of Biological Chemistry·Emile van den AkkerJacques Ghysdael
Jan 27, 2019·Oncotarget·Stefan NagelHans G Drexler
Jul 11, 2006·The Journal of Biological Chemistry·Sashidhar S NakerakantiMaria Trojanowska

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