Dysregulation of renal microRNA expression after deep hypothermic circulatory arrest in rats

European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery
Lei YuChun Wang

Abstract

Acute kidney injury (AKI) is a severe complication of cardiopulmonary bypass-deep hypothermic circulatory arrest (DHCA) surgery. Non-coding microRNAs (miRNAs) are considered as key players in kidney physiology and pathology. However, whether they are implicated in DHCA-induced AKI at the early stage post-surgery is less studied, and requires for further investigation. In this study, kidney tissues were removed at 2 h post-surgery from Sprague-Dawley rats that underwent a 60-min DHCA (18°C), with samples from sham-operated rats as control. Renal RNA isolates were analysed with Affymetrix miRNA microarray 4.0 containing 728 mature rat miRNA probes. Seventy-one miRNAs were down-regulated and 4 were up-regulated in the kidneys of DHCA rats [log2 (fold change, FC) > 1, P < 0.05]. Novel differentially expressed miRNAs, such as miRNA-3068, miR-1949 and miR-3473, were identified in the injured kidney tissues. Putative target genes of the down-regulated miR-30b-5p, miR-199a-5p, miR-148b-3p and miR-10a-3p were subjected to analyses of gene ontology (GO) categories and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The results indicated that these miRNAs targeted a large set of genes involved in essential biological processes re...Continue Reading

References

Mar 9, 1995·The New England Journal of Medicine·M Brezis, S Rosen
Jul 28, 2004·Journal of Molecular and Cellular Cardiology·Gavin Y OuditPeter H Backx
Dec 4, 2004·Journal of Cardiothoracic and Vascular Anesthesia·Gautam M SreeramMark Stafford-Smith
Jan 24, 2006·The Annals of Thoracic Surgery·Anne KuitunenVille Pettilä
Apr 16, 2010·The Journal of Thoracic and Cardiovascular Surgery·Lars EnglbergerHartzell V Schaff
Feb 4, 2012·American Journal of Physiology. Renal Physiology·Rongpei LanManjeri A Venkatachalam
Jun 26, 2012·The Annals of Thoracic Surgery·Go Un RohYon Hee Shim
Apr 30, 2013·BMC Nephrology·Kwon Wook JooKi Young Na
Oct 29, 2013·Chemical Society Reviews·Jinbo LiYan Zhang
Jul 22, 2014·Neuropathology and Applied Neurobiology·Kay Ka-Wai LiHo-Keung Ng
Jun 21, 2015·The Journal of Thoracic and Cardiovascular Surgery·Fang HeXiaojing Jiang
Aug 5, 2015·Briefings in Bioinformatics·Francesco MarabitaRiccardo L Rossi
Aug 10, 2015·The Journal of Thoracic and Cardiovascular Surgery·Yongchao WangQin Fang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis