E-cadherin peptide sequence recognition by anti-E-cadherin antibody

Biochemical and Biophysical Research Communications
K L Lutz, Teruna J Siahaan

Abstract

Cadherins are calcium dependent glycoproteins involved in homophilic cell-cell adhesion. To further elucidate the interaction between cadherins we have developed an immobilized peptide ELISA to quickly scan the extracellular domains of E-cadherin. Peptides displaying antigenic reactivity to anti-E-cadherin antibody are presumably on the surface and thus may be involved in cadherin-cadherin interaction. We have found three peptides from the EC-1 domain which are recognized by the anti-E-cadherin antibody. These peptides are in the two important regions of the EC-1 domain that was deduced from its secondary structure.

Citations

Jul 1, 1996·Pathology, Research and Practice·S VermeulenM Mareel
Dec 1, 1995·Journal of Biomolecular Structure & Dynamics·K L LutzT J Siahaan
Dec 24, 2005·The Journal of Pharmacology and Experimental Therapeutics·Naoki KobayashiTeruna J Siahaan
Jul 16, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·F L M RicciardoloT Mauad
Sep 19, 1997·Journal of Pharmaceutical Sciences·K L Lutz, T J Siahaan

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