E-cadherin/beta-catenin and CD10: a limited immunohistochemical panel to distinguish pancreatic endocrine neoplasm from solid pseudopapillary neoplasm of the pancreas on endoscopic ultrasound-guided fine-needle aspirates of the pancreas

American Journal of Clinical Pathology
Holly BurfordNirag C Jhala

Abstract

Pancreatic endocrine neoplasm (PEN) and solid pseudopapillary neoplasm of the pancreas (SPN) frequently pose diagnostic challenges. We sought to determine which markers could provide the best immunophenotypic characterization of PEN and SPN, allowing separation on limited cytology samples. We retrieved 22 resected PEN (n = 12) and SPN (n = 10) tumors to serve as a training set for the performance of extensive immunohistochemical staining. Based on these results, we selected a subset of antibodies for application to 25 fine-needle aspiration (FNA) samples from PEN (n = 16) and SPN (n = 9). Chromogranin A, synaptophysin, CD56, and progesterone receptor (PR) highlighted PEN cases in the training set; E-cadherin was noted in a membranous pattern. SPN cases were most immunoreactive for alpha(1)-antitrypsin, vimentin, CD10, and PR, with nuclear staining for beta-catenin; E-cadherin did not show a membranous pattern. Among all FNA samples tested, the immunohistochemical staining of E-cadherin (P = .0003), beta-catenin (P = .00004), and CD10 (P = .00006) demonstrated the greatest difference between PEN and SPN. The pattern of E-cadherin/beta-catenin expression was highly specific for distinguishing PEN from SPN. On limited FNA samples,...Continue Reading

References

Feb 1, 1997·Diagnostic Cytopathology·A M LabateM F Zakowski
Apr 12, 2003·The Lancet Oncology·Vincenzo CanzonieriSalvatore Berretta
Sep 7, 2004·Diagnostic Cytopathology·Marino E LeonFernando U Garcia
Feb 26, 2005·Advances in Anatomic Pathology·Edward B StelowMichael W Stanley
Apr 11, 2006·Diagnostic Cytopathology·Edward B StelowMichael W Stanley
Nov 24, 2006·Applied Immunohistochemistry & Molecular Morphology : AIMM·Xiuli LiuNirag Jhala
Mar 6, 2007·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Wendell W TangJingwu Xie
May 9, 2007·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·David S Klimstra
Jun 15, 2007·Cytopathology : Official Journal of the British Society for Clinical Cytology·M B Pitman, V Deshpande
Dec 25, 2007·Advances in Anatomic Pathology·Amy L AdamsNirag C Jhala
Dec 29, 2007·The American Journal of Surgical Pathology·Mona A El-BahrawyGordon Stamp
Aug 19, 2008·Human Pathology·Runjan ChettySima Salahshor

❮ Previous
Next ❯

Citations

Jan 10, 2014·North American Journal of Medical Sciences·Shreyas SaligramMojtaba Olyaee
Sep 18, 2014·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Edoardo VirgilioVincenzo Ziparo
Jun 19, 2013·Abdominal Imaging·Dhakshina Moorthy GaneshanPriya Bhosale
Aug 2, 2013·Internal Medicine·Masakuni FujiiKazuhide Yamamoto
Mar 3, 2017·World Journal of Surgery·Leon NaarNikolaos Arkadopoulos
Mar 1, 2012·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Alice NentwigAnna Oevermann
Jul 1, 2017·Archives of Pathology & Laboratory Medicine·Peyman Dinarvand, Jinping Lai
Apr 6, 2012·Toxicologic Pathology·Christopher KoivistoMark J Hoenerhoff
Apr 24, 2014·Cytopathology : Official Journal of the British Society for Clinical Cytology·E VigliarC Bellevicine
Aug 21, 2020·Cytopathology : Official Journal of the British Society for Clinical Cytology·José Celso ArdenghMarcel Autran Machado
Oct 19, 2013·Oncology Letters·Chiemi SaigoShinji Osada
Nov 1, 2015·Case Reports in Pancreatic Cancer·Jin Bao ZhangCharles J Yeo
Aug 30, 2017·Medical Molecular Morphology·Masahiko TanigawaHirohisa Yano
Oct 31, 2020·Updates in Surgery·Anna Caterina MilanettoClaudio Pasquali
Jun 10, 2021·Head and Neck Pathology·Margaret L ComptonKim A Ely

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adenoma, Islet Cell

Islet Cell Adenoma arises in the islet cells, which are insulin producing cells of the pancreas. These tumors can be either malignant or benign. Discover the latest research on Islet Cell Adenoma here.

Carcinoma, Islet Cell (Keystone)

Islet Cell Carcinoma or Pancreatic Neuroendocrine Tumours arise in the hormone producing cells of the pancreas. These tumors can be either malignant or benign. Discover the latest research on islet cell carcinoma in this feed.

Cell Adhesion Molecules in the Brain

Cell adhesion molecules found on cell surface help cells bind with other cells or the extracellular matrix to maintain structure and function. Here is the latest research on their role in the brain.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Carcinoma, Islet Cell

Islet Cell Carcinoma or Pancreatic Neuroendocrine Tumours arises in the hormone producing cells of the pancreas. These tumors can be either malignant or benign. Discover the latest research on Islet Cell Carcinoma here.