αE-catenin is an autoinhibited molecule that coactivates vinculin.

Proceedings of the National Academy of Sciences of the United States of America
Hee-Jung ChoiWilliam I Weis

Abstract

αE-catenin, an essential component of the adherens junction, interacts with the classical cadherin-β-catenin complex and with F-actin, but its precise role is unknown. αE-catenin also binds to the F-actin-binding protein vinculin, which also appears to be important in junction assembly. Vinculin and αE-catenin are homologs that contain a series of helical bundle domains, D1-D5. We mapped the vinculin-binding site to a sequence in D3a comprising the central two helices of a four-helix bundle. The crystal structure of this peptide motif bound to vinculin D1 shows that the two helices adopt a parallel, colinear arrangement suggesting that the αE-catenin D3a bundle must unfold in order to bind vinculin. We show that αE-catenin D3 binds strongly to vinculin, whereas larger fragments and full-length αE-catenin bind approximately 1,000-fold more weakly. Thus, intramolecular interactions within αE-catenin inhibit binding to vinculin. The actin-binding activity of vinculin is inhibited by an intramolecular interaction between the head (D1-D4) and the actin-binding D5 tail. In the absence of F-actin, there is no detectable binding of αE-catenin D3 to full-length vinculin; however, αE-catenin D3 promotes binding of vinculin to F-actin whe...Continue Reading

References

Jan 24, 1998·The Journal of Biological Chemistry·R B HazanD L Rimm
Mar 22, 2002·The Journal of Biological Chemistry·Sabine PokuttaWilliam I Weis
Jan 2, 2004·Nature·Tina IzardPhilippe R J Bois
Jun 15, 2004·Nature·Constantina BakolitsaRobert C Liddington
Jul 10, 2004·Structure·Robert A BorgonTina Izard
Feb 25, 2005·The Journal of Biological Chemistry·Daniel M CohenSusan W Craig
Jul 1, 2005·Molecular and Cellular Biology·Philippe R J BoisTina Izard
Aug 27, 2005·Development·Rachel E Dawes-HoangEric F Wieschaus
Sep 2, 2005·The Journal of Biological Chemistry·Alexandre R GingrasJonas Emsley
Dec 6, 2005·Cell·Soichiro YamadaW James Nelson
Jan 13, 2006·The Journal of Biological Chemistry·Philippe R J BoisTina Izard
Jan 25, 2006·Journal of Molecular Biology·Deborah F KellyKenneth A Taylor
Nov 1, 2006·The Journal of Biological Chemistry·Hui ChenSusan W Craig
Jun 2, 2007·Annual Review of Cell and Developmental Biology·Sabine Pokutta, William I Weis
Oct 13, 2007·The EMBO Journal·Guy Tran Van Nhieu, Tina Izard
Dec 7, 2007·The Journal of Cell Biology·Jonathan D HumphriesChristoph Ballestrem
Dec 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kentaro Abe, Masatoshi Takeichi
Nov 26, 2008·Nature·Adam C MartinEric F Wieschaus
Dec 17, 2009·Biophysical Journal·Gerold DiezWolfgang H Goldmann
Jan 13, 2010·Cold Spring Harbor Perspectives in Biology·Lawrence Shapiro, William I Weis
May 11, 2010·Nature Cell Biology·Shigenobu YonemuraMai Shibata
May 14, 2010·Proceedings of the National Academy of Sciences of the United States of America·Zhijun LiuChristopher S Chen
Apr 20, 2011·Current Topics in Developmental Biology·Emmanuel Farge
May 24, 2011·Nature Materials·Dhananjay T TambeXavier Trepat
Aug 16, 2011·The Journal of Biological Chemistry·HaJeung ParkTina Izard
Jan 12, 2012·The Journal of Biological Chemistry·Xiao PengKris A DeMali

❮ Previous
Next ❯

Citations

Jan 1, 2013·Protoplasma·Joanne M Leerberg, Alpha S Yap
Feb 19, 2013·Nature Cell Biology·Ridhdhi DesaiUlrich Tepass
Aug 31, 2012·Nature Reviews. Molecular Cell Biology·Aparna Ratheesh, Alpha S Yap
Jan 8, 2013·Nature Structural & Molecular Biology·Erumbi S Rangarajan, Tina Izard
Jul 18, 2012·Proceedings of the National Academy of Sciences of the United States of America·Nicolas BorghiAlexander R Dunn
Dec 26, 2012·The Journal of Biological Chemistry·William A ThomasSylvie Dufour
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Rebecca L DaughertyCara J Gottardi
Aug 1, 2014·Nature Communications·Mingxi YaoJie Yan
Feb 13, 2014·Cell and Tissue Research·Joppe Oldenburg, Johan de Rooij
Apr 9, 2013·Cellular and Molecular Life Sciences : CMLS·Floor Twiss, Johan de Rooij
Jul 26, 2014·Annual Review of Cell and Developmental Biology·D E Leckband, J de Rooij
Aug 12, 2015·The Journal of Cell Biology·Chi-Shuo ChenSergey M Troyanovsky
Oct 28, 2015·The Journal of Cell Biology·Nivetha Kannan, Vivian W Tang
Jan 1, 2013·Tissue Barriers·Verena KüppersDörte Schulte
Mar 26, 2014·Tissue Barriers·Danielle Gulino-Debrac
Jun 7, 2013·Cell Adhesion & Migration·Sylvie DufourJean Paul Thiery
Apr 26, 2016·Scientific Reports·Koichiro MakiTaiji Adachi
Feb 22, 2017·Biology of the Cell·Sophie SluysmansSandra Citi
May 15, 2015·Integrative Biology : Quantitative Biosciences From Nano to Macro·B LadouxR M Mège
Jun 18, 2017·Current Opinion in Cell Biology·Miranda V Hunter, Rodrigo Fernandez-Gonzalez
Jun 28, 2016·Current Biology : CB·Tomohito HigashiAnn L Miller
Mar 16, 2018·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yoshinori HiranoToshio Hakoshima
Nov 17, 2017·Molecular Biology of the Cell·Samantha BarrickDeborah Leckband
Aug 6, 2017·Cold Spring Harbor Perspectives in Biology·Alpha S YapVirgile Viasnoff
Feb 26, 2016·Cell Biology International·Wolfgang H Goldmann
Jan 27, 2018·Scientific Reports·Koichiro MakiTaiji Adachi
Jan 20, 2019·Journal of Cell Science·Herve AlégotKenneth D Irvine
Aug 17, 2017·Nature·Qiangjun ZhouAxel T Brunger
May 16, 2014·Nature Reviews. Molecular Cell Biology·Masatoshi Takeichi
Apr 17, 2013·The Journal of Biological Chemistry·Noboru IshiyamaMitsuhiko Ikura
Jul 19, 2019·Molecular Biology of the Cell·Si Ming PangJie Yan
Dec 10, 2017·EMBO Reports·Shubham DuttaDannel McCollum
Oct 9, 2019·The Journal of Biological Chemistry·Ishani Dasgupta, Dannel McCollum
Dec 14, 2019·ELife·Kendall J LoughScott E Williams
Jun 14, 2015·The Journal of Biological Chemistry·Jing LiEmad Tajkhorshid
Feb 27, 2016·Acta Crystallographica. Section F, Structural Biology Communications·Hyunook KangHee-Jung Choi
Jan 18, 2017·Cold Spring Harbor Perspectives in Biology·René Marc Mège, Noboru Ishiyama

❮ Previous
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