E. coli diversity: low in colorectal cancer.

BMC Medical Genomics
Le TangShu-Lin Liu

Abstract

Escherichia coli are mostly commensals but also contain pathogenic lineages. It is largely unclear whether the commensal E. coli as the potential origins of pathogenic lineages may consist of monophyletic or polyphyletic populations, elucidation of which is expected to lead to novel insights into the associations of E. coli diversity with human health and diseases. Using genomic sequencing and pulsed field gel electrophoresis (PFGE) techniques, we analyzed E. coli from the intestinal microbiota of three groups of healthy individuals, including preschool children, university students, and seniors of a longevity village, as well as colorectal cancer (CRC) patients, to probe the commensal E. coli populations for their diversity. We delineated the 2280 fresh E. coli isolates from 185 subjects into distinct genome types (genotypes) by PFGE. The genomic diversity of the sampled E. coli populations was so high that a given subject may have multiple genotypes of E. coli, with the general diversity within a host going up from preschool children through university students to seniors. Compared to the healthy subjects, the CRC patients had the lowest diversity level among their E. coli isolates. Notably, E. coli isolates from CRC patients...Continue Reading

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Citations

Aug 29, 2020·Expert Review of Anti-infective Therapy·Juraj BosákDavid Šmajs
Nov 9, 2021·Clinical & Experimental Metastasis·Charlotte HenstraAnika Nagelkerke

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Methods Mentioned

BETA
electrophoresis
cleavage profiling

Software Mentioned

SOAPdenovo
SAS
Glimmer
BLAST
GraphPad Prism
Perl scripts

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