E. coli methionine sulfoxide reductase with a truncated N terminus or C terminus, or both, retains the ability to reduce methionine sulfoxide

Protein Science : a Publication of the Protein Society
Sandrine Boschi-MullerGuy Branlant

Abstract

The monomeric peptide methionine sulfoxide reductase (MsrA) catalyzes the irreversible thioredoxin-dependent reduction of methionine sulfoxide. The crystal structure of MsrAs from Escherichia coli and Bos taurus can be described as a central core of about 140 amino acids that contains the active site. The core is wrapped by two long N- and C-terminal extended chains. The catalytic mechanism of the E. coli enzyme has been recently postulated to take place through formation of a sulfenic acid intermediate, followed by reduction of the intermediate via intrathiol-disulfide exchanges and thioredoxin oxidation. In the present work, truncated MsrAs at the N- or C-terminal end or at both were produced as folded entities. All forms are able to reduce methionine sulfoxide in the presence of dithiothreitol. However, only the N-terminal truncated form, which possesses the two cysteines located at the C-terminus, reduces the sulfenic acid intermediate in a thioredoxin-dependent manner. The wild type displays a ping-pong mechanism with either thioredoxin or dithiothreitol as reductant. Kinetic saturation is only observed with thioredoxin with a low K(M) value of 10 microM. Thus, thioredoxin is likely the reductant in vivo. Truncations do no...Continue Reading

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Citations

Jan 15, 2014·Archives of Biochemistry and Biophysics·Eun Hye LeeKwang Yeon Hwang
Jan 15, 2014·Biochimica Et Biophysica Acta·Adrian Drazic, Jeannette Winter
May 31, 2007·Proceedings of the National Academy of Sciences of the United States of America·Zhidong LinW Todd Lowther
Jun 2, 2009·Journal of Bacteriology·Xiangkai Li, Lee R Krumholz
Jan 27, 2011·BMC Systems Biology·Ché S PillayJohann M Rohwer
Nov 7, 2015·PLoS Computational Biology·Kristin J Adolfsen, Mark P Brynildsen
Apr 20, 2017·Nature Reviews. Microbiology·Benjamin EzratyJean-François Collet
Feb 8, 2008·Journal of Molecular Biology·Fanomezana M RanaivosonFrédérique Favier
Dec 1, 2006·The Journal of Biological Chemistry·Nicolas RouhierSandrine Boschi-Muller
Oct 10, 2007·The Biochemical Journal·Hwa-Young Kim, Vadim N Gladyshev
May 15, 2007·The Journal of Biological Chemistry·Adeline GandGuy Branlant
Jul 19, 2020·Free Radical Biology & Medicine·Diego G AriasSergio A Guerrero
Feb 1, 2005·Biochimica Et Biophysica Acta·Sandrine Boschi-MullerGuy Branlant

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