E. coli translation initiation factor IF2--an extremely conserved protein. Comparative sequence analysis of the infB gene in clinical isolates of E. coli

FEBS Letters
S A SteffensenHans Uffe Sperling-Petersen

Abstract

The functionally uncharacterised N-terminal of translation initiation factor IF2 has been found to be extremely variable when comparing different bacterial species. In order to study the intraspecies variability of IF2 the 2670 basepairs nucleotide sequence of the infB gene (encoding IF2) was determined in 10 clinical isolates of E. coli. The N-terminal domains (I, II and III) were completely conserved indicating a specific function of this region of IF2. Only one polymorphic position was found in the deduced 890 amino acid sequence. This Gln/Gly490 is located within the central GTP/GDP-binding domain IV of IF2. The results are further evidence that IF2 from E. coli has reached a highly defined level of structural and functional development.

References

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Citations

Feb 1, 2003·Journal of Molecular Biology·Brian Søgaard LaursenHans Uffe Sperling-Petersen
Oct 7, 2003·Research in Microbiology·Gennadiy KovtunovychNatalia Kozyrovska
Mar 20, 2003·Bioorganic & Medicinal Chemistry Letters·J M EvansC N Parker
Oct 13, 2011·Biochemistry·Louise Carøe Vohlander RasmussenKim Kusk Mortensen
Sep 26, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Brian Søgaard LaursenHans Uffe Sperling-Petersen
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Brian Søgaard LaursenHans Uffe Sperling-Petersen
Jun 22, 2007·Gene·Sheeba RasheediNasreen Z Ehtesham
Jul 2, 2016·Protein Science : a Publication of the Protein Society·Ramachandra DongreHans Wienk
Apr 20, 2005·Journal of Applied Microbiology·X-H Zhang, B Austin
May 27, 2005·Journal of Applied Microbiology·J G Mainil, G Daube
Jun 26, 2020·Genetics and Molecular Biology·Moema Cristina Costa de LimaFernanda Simões de Almeida
Feb 26, 2003·The Journal of Biological Chemistry·Brian Søgaard LaursenDavid W Hoffman
Nov 1, 2020·Applied Microbiology and Biotechnology·Bai-Qing ZhangBang-Ce Ye
Nov 25, 1998·Biochemical and Biophysical Research Communications·J M MorenoH U Sperling-Petersen
Apr 30, 2008·Biochemistry·Louise Carøe Vohlander RasmussenKim Kusk Mortensen

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