E-selectin ligand-1 (ESL-1) is a novel adiponectin binding protein on cell adhesion

Biochemical and Biophysical Research Communications
Hiroyasu YamamotoShinji Kihara

Abstract

Adiponectin (APN) is an adipocyte-derived bioactive molecule with anti-diabetic and anti-atherogenic properties. Although anti-diabetic effects are mostly mediated by the adiponectin receptors AdipoR1 and AdipoR2, the anti-atherogenic mechanisms have not been fully elucidated. In this study, we identified E-selectin ligand (ESL)-1 as a novel APN-binding protein by mass spectrometry analysis of HepG2 cell-derived immunoprecipitant with an anti-APN antibody. Cell adhesion assays using fluorescence-labelled monocyte cell line THP-1 cells and human umbilical vein endothelial cells (HUVECs) revealed that APN-pre-treated THP-1 cells had reduced binding ability to HUVECs. This APN-mediated suppressive effect on monocyte binding to endothelial cells was partially abrogated by targeting ESL-1 with shRNA in THP-1 cells. In addition, serial mutagenesis analysis disclosed that five extracellular amino acids close to the N-terminus of ESL-1 were essential for binding with APN. Our results highlight the fact that interaction between APN and ESL-1 could provide a fundamental mechanism underlying the anti-atherogenic properties of APN.

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Citations

Nov 29, 2016·Scientific Reports·Marion PepinCécile V Denis
Mar 23, 2017·Journal of Atherosclerosis and Thrombosis·Akane MatsumotoShinji Kihara
Nov 17, 2020·Journal of Atherosclerosis and Thrombosis·Makoto FujitaShinji Kihara
Jul 7, 2019·Biochemical and Biophysical Research Communications·Miho SawadaShinji Kihara
Feb 12, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Hiromi UekitaShinji Kihara
Aug 18, 2021·Biochemical and Biophysical Research Communications·Yusi ZhangBoquan Jin

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