E2F-1-deficient NOD/SCID mice developed showing decreased saliva production

Experimental Biology and Medicine
Hikaru Matsui-InoharaHidenobu Senpuku

Abstract

The non-obese diabetic mouse (NOD) is the most characterized model used to study insulin-dependent type 1 diabetes mellitus (IDDM) and Sjoögren's syndrome (SS). In a previous report, we found NOD.E2f1(-/-) mice show a greater progressive development to IDDM and SS compared to NOD mice. Our previous data indicated a progressive decrease in regulatory T cells (CD4(+)CD25(+)) and a decrease in the systemic secretion systems for insulin, and saliva was associated with the progression of IDDM and SS. Therefore, to define the mechanism of early-onset IDDM SS in E2F-1 deficient NOD mice required further investigation by producing E2F-1 deficient NOD/SCID mice in which the T and B cells do not develop. The purpose here was to analyze the essential function of the E2F-1 molecule in the development of IDDM and SS; and the dysfunction of the pancreas islet and salivary gland in the NOD background using NOD/SCID mice. We produced NOD/SCID.E2f1(-/-) mice using homologous recombination; determined diabetes development; measured saliva and insulin production; and performed a histological analysis. The deficient mice showed a decreasing volume of saliva; no infiltration of lymphocytes into salivary glands; no development of diabetes; and no pr...Continue Reading

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Citations

Nov 28, 2012·Pflügers Archiv : European journal of physiology·Keitaro SatohHiroshi Sugiya
Aug 18, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Mohamed EddouksNaoufel Ali Zeggwagh
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Apr 11, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Md Mahmudul HasanTasnuva Sarwar Tunna

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Methods Mentioned

BETA
chips
PCR
electrophoresis
enzyme-linked immunosorbent assay
ELISA
Assay
Protein Assay

Software Mentioned

SPSS
SPSS II

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