E2F-3 accumulation is regulated by polypeptide stability

Oncogene
A M FloresW D Cress

Abstract

E2F is a complex family of transcription factors which appears to regulate the transcription of genes required for the S phase of the mammalian cell cycle. In the present work, we have examined the mechanisms regulating E2F-3 accumulation in mouse fibroblasts. We have determined that E2F-3 DNA binding activity is restricted to the G1/S transition and S phase in both normal BALB/c-3T3 fibroblasts and in an SV40 virus-transformed BALB/c-3T3 derivative. Immunoblot analysis indicates that G0 and G1 cells have little or no E2F-3 polypeptide and that the increase in the DNA binding activity of E2F-3 at the G1/S boundary is reflected by an increase in total E2F-3 protein. In contrast to the E2F-3 polypeptide, RNAse protection assays demonstrate that the E2F-3 mRNA is clearly present in G0 and G1 cells. Finally, pulse/chase experiments indicate that the half-life of E2F-3 is approximately 40-fold greater in cells blocked in S phase relative to asynchronously growing cells. Together, these results indicate that the accumulation E2F-3 at S phase may be regulated, at least in part, at the level of protein stability.

Citations

Oct 13, 2012·The Journal of Biological Chemistry·Lu ChenW Douglas Cress
Jun 15, 2006·The Journal of Biological Chemistry·Jose M RodriguezW Douglas Cress
Mar 13, 2002·Archives of Biochemistry and Biophysics·Yihong MaW Douglas Cress
Jun 10, 2000·Molecular Biology of the Cell·X ZhangW J Pledger
Feb 22, 2002·Oncogene·Rhonda CroxtonW Douglas Cress
May 1, 2002·The Journal of Biological Chemistry·Yiwen He, W Douglas Cress
Mar 4, 2003·The Journal of Biological Chemistry·Yihong MaW Douglas Cress
Jun 14, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Nobuya FujitaShun Ishibashi

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