E2F function in muscle growth is necessary and sufficient for viability in Drosophila

Nature Communications
Maria Paula Zappia, Maxim V Frolov

Abstract

The E2F transcription factor is a key cell cycle regulator. However, the inactivation of the entire E2F family in Drosophila is permissive throughout most of animal development until pupation when lethality occurs. Here we show that E2F function in the adult skeletal muscle is essential for animal viability since providing E2F function in muscles rescues the lethality of the whole-body E2F-deficient animals. Muscle-specific loss of E2F results in a significant reduction in muscle mass and thinner myofibrils. We demonstrate that E2F is dispensable for proliferation of muscle progenitor cells, but is required during late myogenesis to directly control the expression of a set of muscle-specific genes. Interestingly, E2f1 provides a major contribution to the regulation of myogenic function, while E2f2 appears to be less important. These findings identify a key function of E2F in skeletal muscle required for animal viability, and illustrate how the cell cycle regulator is repurposed in post-mitotic cells.

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Citations

Nov 28, 2017·Frontiers in Endocrinology·Pierre-Damien DenechaudAlbert Giralt
Feb 20, 2019·American Journal of Physiology. Endocrinology and Metabolism·Daniil V PopovOlga L Vinogradova
Nov 28, 2018·Nature Communications·Majd M ArissMaxim V Frolov
Jul 13, 2016·Genes & Development·Nicholas J Dyson
May 24, 2019·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Qi Li, Zhengqiang Luo
Sep 16, 2020·BMC Genomics·Natalia RubanovaNadya Morozova
Jul 8, 2020·Cells·Preethi Poovathumkadavil, Krzysztof Jagla
Jul 13, 2021·ELife·Maria Paula ZappiaMaxim V Frolov
Aug 28, 2021·BMC Developmental Biology·Antonio Celestino-MontesFidel de la Cruz Hernández-Hernández
Sep 3, 2021·Developmental Cell·William LodgeLouise Y Cheng
Feb 20, 2020·Seminars in Cell & Developmental Biology·Elena NikonovaMaria L Spletter

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Methods Mentioned

BETA
immunoprecipitation
ChIP
PCR
confocal microscopy

Software Mentioned

Regulatory Sequence Analysis Tools
ImageJ
Graphpad
Image - based Tool for
GraphPad Prism

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