E2f4 and E2f5 are essential for the development of the male reproductive system

Cell Cycle
Paul S DanielianJacqueline A Lees

Abstract

The E2F transcription factors are primarily implicated in the regulation of entry and exit from the cell cycle. However, in vivo studies have established additional roles for E2Fs during organ development and homeostasis. With the goal of addressing the intestinal requirements of E2f4 and E2f5, we crossed mice carrying Vil-cre, E2f4 conditional and E2f5 germline alleles. E2f4 deletion had no detectable effect on intestinal development. However, E2f4f/f;E2f5+/-;Vil-cre males, but not E2f4f/f;Vil-cre littermates, were unexpectedly sterile. This defect was not due to defective spermatogenesis. Instead, the seminiferous tubules and rete testes showed significant dilation, and spermatozoa accumulated aberrantly in the rete testis and efferent ducts. Our data show that these problems result from defective efferent ducts, a tissue whose primary function is to concentrate sperm through fluid absorption. First, Vil-cre expression, and consequent E2F4 loss, was specific to the efferent ducts and not other reproductive tract tissues. Second, the E2f4f/f;E2f5+/-;Vil-cre efferent ducts had completely lost multiciliated cells and greatly reduced levels of critical absorptive cell proteins: aquaporin1, a water channel protein, and clusterin, ...Continue Reading

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Citations

Feb 14, 2016·Cell Cycle·Libor Macurek
Apr 13, 2017·Nature Reviews. Molecular Cell Biology·Nathalie Spassky, Alice Meunier
Feb 14, 2018·Biology of Reproduction·Rex A Hess, Paul S Cooke
Mar 25, 2019·Biology of the Cell·Adel Al JordAlice Meunier
May 28, 2019·Andrology·Laura GirardetClémence Belleannée
Jan 20, 2019·Proceedings of the National Academy of Sciences of the United States of America·Shuiqiao YuanWei Yan
Nov 30, 2019·Cellular and Molecular Life Sciences : CMLS·Anu SironenHannah M Mitchison
Sep 2, 2016·Cold Spring Harbor Perspectives in Biology·Alice Meunier, Juliette Azimzadeh
May 10, 2019·Cell Death and Differentiation·Merit WildungMuriel Lizé
May 5, 2020·Seminars in Cell & Developmental Biology·Michael Lewis, Travis H Stracker
Dec 7, 2020·Seminars in Cell & Developmental Biology·Marie LegendreHannah M Mitchison
Jan 15, 2021·Differentiation; Research in Biological Diversity·Rex A HessBarry T Hinton
Feb 10, 2021·Molecular Human Reproduction·Isabella ApreaHeymut Omran
Mar 5, 2021·Endocrinology·Andrew T MajorCraig A Smith

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Methods Mentioned

BETA
transgenic
transmission electron microscopy
PCR

Software Mentioned

SPOT Advanced
ImageJ

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