EAAC1 glutamate transporter expression in the rat lithium-pilocarpine model of temporal lobe epilepsy

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
Brigitte Voutsinos-PorcheAstrid Nehlig

Abstract

Glutamate excitotoxicity has been involved in the pathophysiology of epilepsy. Normal functioning of glutamate transporters clears the synaptically released glutamate to prevent excitotoxic neuronal death. Using densitometric immunohistochemical analysis, we examined the temporal expression of the neuronal glutamate transporter (EAAC1) in the lithium-pilocarpine rat model of temporal lobe epilepsy. During the acute period of lithium-pilocarpine-induced status epilepticus, EAAC1 transporter expression increased in the pyramidal neurons of cornus ammonis (CA)1, CA2 and CA3 (fields of the hippocampus), in dentate gyrus (DG) granule cells and in olfactory tubercle (Tu). During the latent period, EAAC1 expression was strongly expressed in the DG granular and molecular layers, Tu, cerebral cortex and septum, and went back to control levels in CA1, CA2 and CA3 layers. The overexpression of EAAC1 occurred mainly in structures prone to develop Fluoro-Jade-B-positive degenerating neurons. It is, however, not clear to what extent the overexpression of EAAC1 contributes to epileptogenesis and in which area it may represent a preventive or compensatory or response to injury.

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Citations

Dec 22, 2007·The Journal of Biological Chemistry·Yiting LiuJeffrey D Rothstein
Oct 29, 2013·Cellular and Molecular Life Sciences : CMLS·Massimiliano G BianchiOvidio Bussolati
Jan 27, 2009·Neurochemical Research·Taku DoiL James Willmore
Jan 14, 2009·Biochemical and Biophysical Research Communications·Lili CaoZhaofu Chi
May 23, 2007·Neurochemistry International·Amanda L Sheldon, Michael B Robinson
Apr 10, 2007·Epilepsy Research·Kristopher J BoughRaymond Dingledine
Sep 25, 2007·Journal of Neurochemistry·Daniel KondziellaUrsula Sonnewald
Nov 16, 2019·International Journal of Molecular Sciences·Anna R Malik, Thomas E Willnow
Jun 6, 2007·Neurochemical Research·Zhao Wei LiuZhuo Yang
Nov 25, 2019·Epilepsy & Behavior : E&B·Victor Rodrigues SantosOlagide Wagner de Castro

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Methods Mentioned

BETA
antisense oligonucleotide
surgical resections

Software Mentioned

Visilog
Image J
SPSS®

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