PMID: 2495845Feb 1, 1989Paper

Early and late appearance of neurofilament phosphorylation events in nerve regeneration

Brain Research Bulletin
D DahlA Bignami

Abstract

Neurofilament phosphorylation in regenerating rat sciatic nerve was studied by indirect immunofluorescence with monoclonal antibodies reacting with phosphorylated epitopes of the 2 large polypeptides of the neurofilament protein triplet (NF 150K, NF 200K). One group of antibodies decorated axons early in the process. In fact, no differences were seen in double labeled sections between these antibodies and polyclonal neurofilament antibodies as to their reactivity with the distal stump of transected sciatic nerves. Another group stained axons after they had completed their elongation, i.e., after they had reached the distal part of the denervated sciatic nerve. In general, the epitopes recognized by antibodies in this group appeared more sensitive to phosphatase digestion as compared to the first group. Furthermore, there was a good correlation between the thickness of the regenerated axons and staining with these monoclonal antibodies. Thick axons (like those observed in normal nerves) were stained, while bundles of thin axons remained unstained. Monoclonal II32 stained regenerated axons in a remarkable segmental pattern. With this antibody, continuous decoration of the axons was still not observed 7 weeks after transection, th...Continue Reading

References

Dec 15, 1977·The Journal of Comparative Neurology·D Dahl, A Bignami
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Jan 1, 1986·Experimental Cell Research·D Dahl, A Bignami
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Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L A Sternberger, N H Sternberger

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Citations

Apr 1, 1992·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·D DahlA Bignami
Aug 19, 1999·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·N B ChauhanT Khan
Jan 29, 2000·Brain : a Journal of Neurology·G LovasS Komoly
Apr 13, 2007·Experimental Cell Research·Amar ThyagarajanBen G Szaro

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