Early and transient cytotoxic response of peritoneal cells from Fasciola hepatica-infected rats

Veterinary Research
Pierre SibilleChantal Boulard

Abstract

Experimental infection by F. hepatica was performed on rats. Early recruitment of the peritoneal cell population was observed and revealed transient parasite-killing activity, preceded and followed by a state of total unresponsiveness. The activation peaked at seven days post-infection (dpi) and was characterised by a massive peritoneal cell recruitment, a strong superoxide anion and nitric oxide (NO) production, that were coincident with the fasciolicide activity of these cells, as monitored by an in vitro decrease of juvenile fluke viability in a conditioned medium. The addition of L-NG-monomethyl arginine (LNMMA) to cell cultures abrogated both fasciolicide activity and NO production. Parasites started to die when NO production exceeded 25 microM and all juvenile flukes were killed by a 90 microM NO exposition (Lethal Dose 50 between 45.8 and 50.3 microM, 95% fiducial limits). However, when rat peritoneal cells were cultured in the presence of either infected or control rat serum, juvenile flukes were much more resistant to the oxidative burst, despite a massive attachment of rat peritoneal cells to the parasite tegument. These data suggest that a transient control of fasciolosis may take place in the peritoneum following th...Continue Reading

Citations

Feb 13, 2010·Journal of Biomedicine & Biotechnology·Emmanuelle Moreau, Alain Chauvin
Oct 18, 2016·Oxidative Medicine and Cellular Longevity·Somayeh BahramiHossein Hamidinejat
Aug 22, 2009·Toxicology Mechanisms and Methods·Ewa SiemieniukElżbieta Skrzydlewska
Sep 25, 2017·Tropical Animal Health and Production·Somayeh BahramiSaeed Sardarifar
Sep 8, 2018·Parasites & Vectors·Raúl Pérez-CaballeroÁlvaro Martínez-Moreno
Jan 25, 2006·Experimental Parasitology·Lidia KolodziejczykElzbieta Skrzydlewska

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