DOI: 10.1101/19002550Nov 25, 2019Paper

Early assessment of molecular progression and response by whole-genome circulating tumor DNA in advanced solid tumors

MedRxiv : the Preprint Server for Health Sciences
A. A. DavisYoung Kwang Chae

Abstract

Treatment response assessment for patients with advanced solid tumors is complex and existing methods require greater precision. Current guidelines rely on imaging, which has known limitations, including the time required to show a deterministic change in target lesions. Serial changes in whole-genome (WG) circulating tumor DNA (ctDNA) were used to assess response or resistance to treatment early in the treatment course. 96 patients with advanced cancer were prospectively enrolled (91 analyzed and 5 excluded), and blood was collected before and after initiation of a new, systemic treatment. Plasma cell-free DNA libraries were prepared for either WG or WG bisulfite sequencing. Longitudinal changes in the fraction of ctDNA were quantified to retrospectively identify molecular progression (MP) or major molecular response (MMR). Study endpoints were concordance with first follow-up imaging (FFUI) and stratification of progression-free survival (PFS) and overall survival (OS). Patients with MP (n=13) had shorter PFS (median 62d vs. 310d) and OS (255d vs. not reached). Sensitivity for MP to identify clinical progression was 54% and specificity was 100%. MP calls were from samples taken a median of 28d into treatment and 39d before FF...Continue Reading

Related Concepts

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology
Daniel D Von HoffRobert Penny
Mayo Clinic Proceedings
L E GibsonH O Perry
Thrombosis Research
Anna FalangaMarina Marchetti
The American Journal of Hospice & Palliative Care
Wael LasheenDilara Seyidova Khoshknabi
© 2021 Meta ULC. All rights reserved