Early attachment sites for Shiga-toxigenic Escherichia coli O157:H7 in experimentally inoculated weaned calves.

Applied and Environmental Microbiology
Evelyn A Dean-NystromJoachim F L Pohlenz

Abstract

Weaned 3- to 4-month-old calves were fasted for 48 h, inoculated with 10(10) CFU of Shiga toxin-positive Escherichia coli (STEC) O157:H7 strain 86-24 (STEC O157) or STEC O91:H21 strain B2F1 (STEC O91), Shiga toxin-negative E. coli O157:H7 strain 87-23 (Stx(-) O157), or a nonpathogenic control E. coli strain, necropsied 4 days postinoculation, and examined bacteriologically and histologically. Some calves were treated with dexamethasone (DEX) for 5 days (3 days before, on the day of, and 1 day after inoculation). STEC O157 bacteria were recovered from feces, intestines, or gall bladders of 74% (40/55) of calves 4 days after they were inoculated with STEC O157. Colon and cecum were sites from which inoculum-type bacteria were most often recovered. Histologic lesions of attaching-and-effacing (A/E) O157(+) bacteria were observed in 69% (38/55) of the STEC O157-inoculated calves. Rectum, ileocecal valve, and distal colon were sites most likely to contain A/E O157(+) bacteria. Fecal and intestinal levels of STEC O157 bacteria were significantly higher and A/E O157(+) bacteria were more common in DEX-treated calves than in nontreated calves inoculated with STEC O157. Fecal STEC O157 levels were significantly higher than Stx(-) O157, ...Continue Reading

References

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Dec 26, 2006·Applied and Environmental Microbiology·Ji Youn LimCarolyn J Hovde

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Citations

Jul 23, 2014·BioMed Research International·Sangeetha Ananda Baskaran, Kumar Venkitanarayanan
Apr 10, 2014·Tropical Animal Health and Production·Cristine CervaFabiana Quoos Mayer
Jan 29, 2011·Journal of Biomedicine & Biotechnology·Krystle L Mohawk, Alison D O'Brien
Sep 4, 2008·Infection and Immunity·Pablo NartJ Christopher Low

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