Early cell-wall modifications of maize cell cultures during habituation to dichlobenil

Journal of Plant Physiology
María de CastroJ L. Acebes

Abstract

Studies involving the habituation of plant cell cultures to cellulose biosynthesis inhibitors have achieved significant progress as regards understanding the structural plasticity of cell walls. However, since habituation studies have typically used high concentrations of inhibitors and long-term habituation periods, information on initial changes associated with habituation has usually been lost. This study focuses on monitoring and characterizing the short-term habituation process of maize (Zea mays) cell suspensions to dichlobenil (DCB). Cellulose quantification and FTIR spectroscopy of cell walls from 20 cell lines obtained during an incipient DCB-habituation process showed a reduction in cellulose levels which tended to revert depending on the inhibitor concentration and the length of time that cells were in contact with it. Variations in the cellulose content were concomitant with changes in the expression of several ZmCesA genes, mainly involving overexpression of ZmCesA7 and ZmCesA8. In order to explore these changes in more depth, a cell line habituated to 1.5μM DCB was identified as representative of incipient DCB habituation and selected for further analysis. The cells of this habituated cell line grew more slowly an...Continue Reading

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Citations

Mar 5, 2015·Journal of Integrative Plant Biology·Hugo MélidaAntonio Encina
May 6, 2017·Journal of Integrative Plant Biology·María de CastroPenélope García-Angulo
Jan 14, 2018·Planta·J Mabel Hernández-AltamiranoPenélope García-Angulo
Feb 22, 2018·Physiologia Plantarum·Romina Martínez-RubioAnna Kärkönen
Aug 29, 2021·Plants·Alfonso Gonzalo De la RubiaPenélope García-Angulo

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