Early changes in gene expression induced by acute UV exposure in leaves of Psychotria brachyceras, a bioactive alkaloid accumulating plant.

Molecular Biotechnology
Naíla Cannes do NascimentoArthur Germano Fett-Neto

Abstract

UV-B radiation can damage biomolecules, such as DNA, RNA, and proteins, halting essential cellular processes; this damage is partly due to ROS generation. Plant secondary metabolites may protect against UV-B. Psychotria brachyceras Müll. Arg. (Rubiaceae), a subtropical shrub, produces brachycerine, a monoterpene indole alkaloid mainly accumulated in leaf tissues, which displays antioxidant and antimutagenic activities. Exposure of P. brachyceras cuttings to UV-B radiation significantly increases leaf brachycerine concentration. It has been suggested that this alkaloid might contribute to protection against UV-B damage both through its quenching activity on ROS and as UV shield. To identify differentially expressed genes of P. brachyceras in response to UV-B and investigate a possible influence of this stimulus on putative brachycerine-related genes, suppressive subtractive hybridization was applied. Complementary DNA from UV-B-treated leaves for 24 h was used as tester, and cDNA from untreated leaves, as driver. After BLASTX alignments, 134 sequences matched plant genes. Using quantitative RT-PCR, selected genes potentially related to brachycerine showed significant increases in transcription after UV-B exposure: tryptophan dec...Continue Reading

References

Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·L DiatchenkoP D Siebert
May 26, 2001·Journal of Natural Products·V A KerberA T Henriques
Sep 25, 2001·Micron : the International Research and Review Journal for Microscopy·F Hollósy
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 1, 1995·The Plant Cell·R. Green, R. Fluhr
Mar 28, 2003·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Martyn M CaldwellManfred Tevini
Dec 5, 2003·Photochemistry and Photobiology·Tatiana S GregianiniArthur G Fett-Neto
Dec 18, 2003·Biotechnology Letters·Bo-Ra KimYung-Jin Chang
Dec 19, 2003·Plant Physiology·Hanns Frohnmeyer, Dorothee Staiger
Dec 22, 2004·Journal of Chemical Ecology·Tatiana Schäffer GregianiniArthur Germano Fett-Neto
Aug 9, 2006·Journal of Plant Physiology·Christian ZinserWerner Rau
Nov 7, 2006·Plant Cell Reports·Jong-Sug ParkKang-Jin Cho
Nov 2, 2007·Environmental and Molecular Mutagenesis·Naíla Cannes do NascimentoJenifer Saffi
Apr 29, 2008·Journal of Molecular Signaling·Shilpa Ramani, Chelliah Jayabaskaran
Apr 30, 2009·Annual Review of Plant Biology·Gareth I Jenkins

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Citations

Sep 26, 2013·Biotechnology Letters·Hélio Nitta MatsuuraArthur Germano Fett-Neto
Sep 2, 2021·Horticulture Research·Prisca MeyerBarbara De Coninck

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