Early cytokine gene expression in mouse CNS after peripheral nerve lesion

Neuroscience Letters
Nurcan UçeylerC Sommer

Abstract

The generation and maintenance of pain after peripheral nerve injury are thought to be influenced by cytokine signaling. Chronic constriction injury (CCI) to mouse sciatic nerve leads to an early local upregulation of pro-inflammatory cytokines already 1 h after the lesion. The early regulation of cytokines in pain related CNS areas is largely unknown. We investigated cytokine regulation in the lumbar spinal cord, hypothalamus, thalamus, hippocampus, and frontal cortex in C57Bl/6J mice after lesioning the right sciatic nerve by CCI. The gene expression of tumor necrosis factor-alpha (TNF), interleukin (IL)-1beta, IL-4, and IL-10 was analyzed by quantitative real-time-PCR from 1 to 12 h after surgery or until values were back to baseline. CCI led to an early downregulation of TNF and IL-1beta mRNA in distinct brain areas and in the lumbar spinal cord with a maximum decrease within the first 6h after CCI. The reduction of TNF mRNA was inhibited by the NMDA receptor antagonist (+)-MK-801, while the calpain inhibitor MDL-28170 had no effect. Our results suggest an early cytokine regulation in the CNS after peripheral nerve lesion, which is opposite in direction to that in the periphery and which is partly mediated by the NMDA recep...Continue Reading

References

Jul 20, 2002·Regional Anesthesia and Pain Medicine·William C CoveyRobert N Spengler
Dec 31, 2003·Brain Research Bulletin·Alessandro BartolomucciRobert Dantzer
Aug 19, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Eiko K de JongKnut Biber

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Citations

Sep 28, 2012·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Pablo AndradeVeerle Visser-Vandewalle
May 12, 2011·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Pablo AndradeGovert Hoogland
Dec 4, 2013·Annals of Biomedical Engineering·Nassir Mokarram, Ravi V Bellamkonda
Dec 14, 2011·PloS One·Nurcan ÜçeylerClaudia Sommer
Aug 26, 2014·Neuroscience and Biobehavioral Reviews·Ipek YalcinMichel Barrot
Jul 23, 2013·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Ankesh KukkarAmteshwar Singh Jaggi
Mar 7, 2013·Pain Medicine : the Official Journal of the American Academy of Pain Medicine·Masae AraiAtsuhiro Sakamoto
Sep 19, 2012·Cytokine & Growth Factor Reviews·Pía M VidalSven Hendrix
Apr 12, 2008·Neuroscience Letters·Nurcan Uçeyler, Claudia Sommer
Jul 25, 2012·Annals of the New York Academy of Sciences·Adriana del ReyHugo O Besedovsky
Jun 12, 2016·Chemico-biological Interactions·Daniela T Longhi-BalbinotWaldiceu A Verri
Jul 27, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nurcan UçeylerClaudia Sommer
Jul 30, 2017·Histochemistry and Cell Biology·Marcela KohoutkováPetr Dubový
Oct 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Arkady KhoutorskyNahum Sonenberg

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