Early cytoplasmic acidification in retinamide-mediated apoptosis of human promyelocytic leukemia cells

Biochemical and Biophysical Research Communications
D AngoliE Wanke

Abstract

The retinamide-mediated apoptosis of promyelocytic cells was investigated using pHi- and DNA-sensitive fluorescent probes (BCECF and Hoechst 33342). Acidification and apoptosis were observed during prolonged (0-12 h) exposure to retinamide (1 microM), but were absent in a retinamide-resistant clone. The analysis of experiments performed by simultaneous staining with the two dyes showed that acidification and apoptosis are correlated; the half-time for acidification is about 5 h while that for apoptosis is 80% longer. On the whole, these results suggest that apoptosis is preceded by an early mechanism of acidification in human leukemia cells treated by a retinoid of clinical interest in cancer chemo prevention and therapy.

Citations

Aug 30, 2000·Oncogene·S PanigoneD Delia
Oct 19, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ming ZhengRui-Ping Xiao
Aug 15, 2001·American Journal of Physiology. Cell Physiology·H BarrièreP Poujeol
Oct 3, 1999·Archives of Pathology & Laboratory Medicine·X ZhangF Kiechle

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