Early cytoskeletal rearrangement during dendritic cell maturation enhances synapse formation and Ca(2+) signaling in CD8(+) T cells

European Journal of Immunology
Marco AverbeckChristian Termeer

Abstract

The interplay between dendritic cells (DC) and T cells is a dynamic process critically depending on DC maturation. Ca(2+) influx is one of the initial events occurring during DC/T cell contacts. To determine how DC maturation influences DC/T cell contacts, time-lapse video microscopy was established using TCR-transgenic CD8(+) T cells from P14 mice. DC maturation shifted DC/T cell contacts from short-lived interactions with transient Ca(2+) influx in T cells to long-lasting interactions and sustained Ca(2+) influx of 30 min and more. Follow-up of DC/T cell interactions after 2 h using confocal microscopy revealed that long-lasting Ca(2+) responses in T cells were preferentially associated with the formation of an immunological synapse involving CD54 and H2-K(b) at the DC/T cell interface. Such synapse formation preceded MHC or B7 up-regulation, since DC developed into potent Ca(2+) stimulators 7 h after initiation of maturation. Instead, the enhanced capacity of 7 h-matured DC to induce sustained Ca(2+) responses in CD8(+) T cells is critically dependent on the polarization and rearrangement of the cytoskeleton, as shown by Clostridium difficile toxin B inhibitor experiments. These data indicate that already very early after re...Continue Reading

Citations

Aug 24, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shu-Yan DaiMitsuomi Hirashima
Apr 11, 2006·Current Opinion in Immunology·Saso Cemerski, Andrey Shaw
Feb 1, 2006·Immunobiology·Leandro J CarreñoAlexis M Kalergis
Jan 6, 2006·Nihon Rinshō Men'eki Gakkai kaishi = Japanese journal of clinical immunology·Akira YamauchiMitsuomi Hirashima

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