Early decrease in basophil sensitivity to Ara h 2 precedes sustained unresponsiveness after peanut oral immunotherapy

The Journal of Allergy and Clinical Immunology
Sarita U PatilWayne G Shreffler

Abstract

Only some peanut-allergic subjects undergoing oral immunotherapy (OIT) achieve sustained clinical response. Basophil activation could provide a functional surrogate of efficacy. We hypothesized that changes in basophil sensitivity and AUC to the immunodominant allergen Ara h 2 correlate with clinical responses to OIT. Peanut-allergic children, aged 7-13, enrolled in a single-center, open-label peanut OIT trial. Specific immunoglobulins were measured throughout OIT. Peripheral blood from multiple time points was stimulated in vitro with peanut allergens for flow cytometry assessment of the percent CD63hi activated basophils. Twenty-two of 30 subjects were successfully treated by OIT; post-avoidance, 9 achieved sustained unresponsiveness (SU) and 13 had transient desensitization (TD). Basophil sensitivity, measured by ED50, to Ara h 2 stimulation decreased from baseline in SU (post-OIT p= 0.0041, post-avoidance p=0.0011). At 3 months of OIT, basophil sensitivity in SU decreased from baseline compared to TD (median 18-fold vs. 3-fold, p=0.01) with an ROC of 0.84 and optimal fold-change of 4.9. Basophil AUC, measured by area-under-the-curve, to Ara h 2 was suppressed post-OIT equally in SU and TD (p=0.4). After avoidance, basophil ...Continue Reading

Citations

Mar 19, 2020·Bioscience Reports·Hiu Yan LamKwang Seok Ahn
Dec 5, 2019·Current Allergy and Asthma Reports·LaKeya C HardyMichael D Kulis
Mar 4, 2020·Immunity, Inflammation and Disease·Teruaki MatsuiKomei Ito
Apr 22, 2020·Current Allergy and Asthma Reports·Meera PatrawalaBrian Vickery
Sep 6, 2020·Current Opinion in Allergy and Clinical Immunology·Hope JinAnna Nowak-Wegrzyn
Nov 24, 2020·Frontiers in Immunology·Ann-Marie Malby SchoosWillem van de Veen
Jan 9, 2021·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Suzanne M BarshowEdwin H Kim
Dec 29, 2020·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Rachel L PetersAlexandra F Santos
Jan 1, 2021·Frontiers in Immunology·Anuya ParanjapeStephen J Galli
Nov 6, 2020·The Journal of Allergy and Clinical Immunology·Didier G EboVito Sabato
Apr 17, 2021·Archives de pédiatrie : organe officiel de la Sociéte française de pédiatrie·M SabouraudC Braun
Mar 27, 2021·Current Opinion in Allergy and Clinical Immunology·Amber N PepperThomas B Casale
Mar 15, 2021·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Nicole Ramsey, M Cecilia Berin
Aug 11, 2021·Current Opinion in Allergy and Clinical Immunology·Tarun Keswani, Sarita U Patil
Sep 17, 2021·The Journal of Allergy and Clinical Immunology. in Practice·Alexandra F SantosHugh A Sampson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basophils

Basophils are myeloid cells with a high affinity IgE receptor and is involved in inflammatory responses during allergy. Discover the latest research on Basophils here.

Related Papers

Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology
Asako NozawaKazuyunki Kurihara
Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
Kelly G OrgelMichael Kulis
The Journal of Allergy and Clinical Immunology
Brian P VickeryA Wesley Burks
Allergy
D A Moneret-Vautrin, Lina Mayorga
© 2021 Meta ULC. All rights reserved