Early detection of hepatitis B surface antigen and detection of HBsAg mutants: a comparison of five assays

Vox Sanguinis
H L ZaaijerM Koot

Abstract

Assays for the detection of hepatitis B surface antigen (HBsAg) face two challenges: the emergence of sensitive techniques for detection of hepatitis B virus DNA and the existence of HBsAg mutant hepatitis B viruses. We studied the sensitivity of five modern assays for detection of HBsAg. The sensitivity of two mini robot-based assays--IMx HBsAg V2 and Vidas HBsAg--and three enzyme-linked immunosorbent assays (ELISAs) with microtitre plate format--Hepanostika Uni-Form II v1.2, Monolisa Ag HBs Plus and HBsAg Test System 3--was compared testing 11 HBsAg seroconversion series, serial dilutions prepared from three HBsAg standards and nine HBsAg mutant samples. Overall, the IMx HBsAg V2 assay showed the highest sensitivity. It outperformed the Vidas HBsAg in analytical sensitivity and in detection of HBsAg mutants. Among the microtitre plate ELISAs, the Monolisa Ag HBs Plus outperformed the HBsAg Test System 3 in detection of HBsAg mutants and it surpassed the Hepanostika Uni-Form II v1.2 in analytical sensitivity.

References

Aug 10, 1999·Journal of Medical Virology·P F ColemanI K Mushahwar

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Citations

Jun 29, 2002·Journal of Clinical Microbiology·Carla Osiowy
Sep 11, 2013·BMC Research Notes·Beatrice Mukami MuriukiSamoel Ashimosi Khamadi
Mar 1, 2008·Expert Opinion on Medical Diagnostics·Prista Charuworn, Ramsey Cheung
Mar 26, 2004·Journal of Medical Virology·M H G M Koppelman, H L Zaaijer
Jun 8, 2006·Vox Sanguinis·L Comanor, P Holland
Dec 14, 2005·Journal of Hepatology·Angelos HatzakisCatherine Haida
Jul 14, 2012·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Seyed Moayed AlavianSeyed Mohammad Jazayeri
Aug 4, 2016·PloS One·Rachel Louise KnowlesUNKNOWN UK Collaborative Study of Congenital Heart Defects (UKCSCHD)
Feb 24, 2006·Emerging Infectious Diseases·Paul F Coleman
Sep 19, 2006·World Journal of Gastroenterology : WJG·Azam RoohiFazel Shokri

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