Early Detection of Preeclampsia Using Circulating Small non-coding RNA

Scientific Reports
Liron YoffeNoam Shomron

Abstract

Preeclampsia is one of the most dangerous pregnancy complications, and the leading cause of maternal and perinatal mortality and morbidity. Although the clinical symptoms appear late, its origin is early, and hence detection is feasible already at the first trimester. In the current study, we investigated the abundance of circulating small non-coding RNAs in the plasma of pregnant women in their first trimester, seeking transcripts that best separate the preeclampsia samples from those of healthy pregnant women. To this end, we performed small non-coding RNAs sequencing of 75 preeclampsia and control samples, and identified 25 transcripts that were differentially expressed between preeclampsia and the control groups. Furthermore, we utilized those transcripts and created a pipeline for a supervised classification of preeclampsia. Our pipeline generates a logistic regression model using a 5-fold cross validation on numerous random partitions into training and blind test sets. Using this classification procedure, we achieved an average AUC value of 0.86. These findings suggest the predictive value of circulating small non-coding RNA in the first trimester, warranting further examination, and lay the foundation for producing a nov...Continue Reading

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Citations

Sep 13, 2019·Molecular Biology Reports·Batool TeimooriSaeedeh Salimi
May 1, 2020·Acta Obstetricia Et Gynecologica Scandinavica·Ida Catharina PüschlBas B Van Rijn
Apr 10, 2019·Acta Paediatrica·Mark Hanson
Jan 9, 2019·Frontiers in Physiology·Rafael Tomoya MichitaJosé Artur Bogo Chies
Aug 27, 2019·Frontiers in Neurology·Dalia YousifTimo Siepmann
Mar 23, 2019·Frontiers in Neurology·Greig JoilinMajid Hafezparast
Nov 30, 2019·Development·Margherita Y Turco, Ashley Moffett
Mar 7, 2021·International Journal of Molecular Sciences·Asghar AliSiriluck Ponsuksili
Aug 26, 2021·Expert Review of Molecular Diagnostics·Danai MavreliAggeliki Kolialexi

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Methods Mentioned

BETA
PCR
RNA-Seq
Assay

Software Mentioned

glmulti
Ensembl
DESeq2
fastq
SVA
R
glm
mcf

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