Early developmental alterations of low-Mg2+ -induced epileptiform activity in the intact corticohippocampal formation of the newborn mouse in vitro

Brain Research
Jochen MoserHeiko J Luhmann

Abstract

The generation, propagation and pharmacological properties of low-Mg2+ -induced epileptiform activity were examined in the intact corticohippocampal formation (CHF) of the newborn (P0-4) mouse in vitro. Multi-site field potential recordings in dentate gyrus (DG), CA3, CA1, entorhinal cortex (EC) and temporal cortex (TC) revealed in 0.2 mM Mg2+ -containing ACSF a stable pattern of spontaneous epileptiform activity consisting of recurrent ictal-like events (ILEs) and interictal events (IEs). Although this activity could be consistently observed as early as P0, ILEs were smaller in amplitude, less frequent and showed a slower onset in P0-2 as compared to P3-4 animals. In all age groups, epileptiform events were largest in CA3 and smallest in EC and TC. A specific pacemaker region could not be identified since ILEs appeared simultaneously at all recording sites. Reducing the extracellular Mg2+ concentration to 0.1 mM or nominally zero caused an increase in ILE frequency. Pharmacological studies in the P3-4 age group with 0.2 mM Mg2+ revealed a complete blockade of the ILEs by an NMDA receptor antagonist and a pronounced suppression of epileptiform activity by an AMPA/kainate antagonist. Application of a GABA-A receptor antagonist i...Continue Reading

References

Jul 14, 1995·Brain Research. Developmental Brain Research·T GloveliU Heinemann
Nov 1, 1996·American Journal of Perinatology·H Osiovich, K Barrington
Feb 26, 1999·Journal of Neurophysiology·R KhazipovY Ben-Ari
Nov 27, 1999·Developmental Neuroscience·I KhalilovR Khazipov
Jul 18, 2000·Epilepsia·S LiptákováS L Moshé
Sep 11, 2001·Brain Research·L AniksztejnA Represa
Jun 18, 2002·Science·Xavier LeinekugelGyörgy Buzsáki
Sep 5, 2002·Nature Reviews. Neuroscience·Yehezkel Ben-Ari
Sep 7, 2002·Mental Retardation and Developmental Disabilities Research Reviews·Linda D Cowan
Oct 10, 2002·The European Journal of Neuroscience·P P QuilichiniH Gozlan
Mar 13, 2003·Cerebral Cortex·Werner Kilb, Heiko J Luhmann
Sep 18, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ilgam KhalilovRoustem Khazipov

❮ Previous
Next ❯

Citations

Apr 5, 2014·PLoS Genetics·Francisco J ArjonaJoost G J Hoenderop
Oct 6, 2007·Annals of Neurology·Volodymyr I DzhalaKevin J Staley
Jan 18, 2018·Neuromolecular Medicine·Pradeep Punnakkal, Deity Dominic

❮ Previous
Next ❯

Related Concepts

Related Feeds

Barrel cortex

Here is the latest research on barrel cortex, a region of somatosensory and motor corticies in the brain, which are used by animals that rely on whiskers for world exploration.

Amygdala: Sensory Processes

Amygdalae, nuclei clusters located in the temporal lobe of the brain, play a role in memory, emotional responses, and decision-making. Here is the latest research on sensory processes in the amygdala.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Jie JinDavid E Clapham
American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation
Henrik DimkeRené J Bindels
Physiology
Joost G Hoenderop, René J Bindels
© 2021 Meta ULC. All rights reserved