Early diagnosis of human immunodeficiency virus-1 infection in infants with the NucliSens EasyQ assay on dried blood spots

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
R R LilianG G Sherman


More routine laboratories in South Africa are equipped to perform quantitative than qualitative HIV viral detection assays. The accessibility of early infant diagnosis would be improved if a quantitative viral load (VL) assay performed on dried blood spots (DBS) could accurately diagnose HIV-infection. The VL assay routinely used in the country has not previously been assessed on DBS for early infant diagnosis. To determine the accuracy of the NucliSens EasyQ assay (bioMerieux, Lyon, France) on DBS for early infant diagnosis of HIV in a subtype C-infected population. Stored DBS samples collected from children presenting for HIV testing were analyzed. DBS EasyQ VL results were compared to the child's HIV status as determined by a whole blood HIV DNA PCR result. The EasyQ assay was performed on 235 stored DBS samples from 71 HIV-infected and 164 HIV-uninfected children. Of the 216 infants (children aged 12 months or less) tested, all 52 HIV-infected infants were detected (sensitivity of 100%). Six of 164 HIV-uninfected infants yielded false positive results (specificity 96.3%). All false positive and six of the true positive infants had VL<3.7 log IU/ml. These 12 (5.6%) infants would require repeat testing to differentiate true f...Continue Reading


Dec 6, 2003·Journal of Acquired Immune Deficiency Syndromes : JAIDS·John S LambertLynne M Mofenson
May 26, 2005·Journal of Virological Methods·Sumonmal UttayamakulRuengpung Sutthent
Nov 21, 2008·The New England Journal of Medicine·Avy ViolariUNKNOWN CHER Study Team
Jun 9, 2009·PloS One·Nishaki MehtaMohan Somasundaran

❮ Previous
Next ❯


Jun 28, 2011·Analytical and Bioanalytical Chemistry·Julien DéglonChristian Staub
Sep 13, 2012·Sensors·Chia-Chen ChangChii-Wann Lin
Dec 25, 2013·The American Journal of Tropical Medicine and Hygiene·Pieter W SmitPaul N Newton
Oct 20, 2012·Journal of the International AIDS Society·Teri RobertsNathan Ford
Dec 24, 2013·Analytical Biochemistry·Grace WuMuhammad H Zaman
Sep 25, 2010·Journal of Pharmaceutical and Biomedical Analysis·Julien DéglonChristian Staub
Sep 25, 2010·Journal of Virological Methods·Marco Rossi de GasperisFrancesco Callea
Apr 19, 2017·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Divya MallampatiMartina Penazzato
Oct 15, 2019·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Robert LuoLara Vojnov
Sep 26, 2013·Expert Review of Molecular Diagnostics·Thomas BourletBruno Pozzetto
Mar 25, 2019·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Ahmad Haeri MazanderaniUNKNOWN LEOPARD Study Team

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Landau-Kleffner Syndrome

Landau Kleffner syndrome (LKS), also called infantile acquired aphasia, acquired epileptic aphasia, or aphasia with convulsive disorder, is a rare childhood neurological syndrome characterized by the sudden or gradual development of aphasia (the inability to understand or express language) and an abnormal electroencephalogram. Discover the latest research on LKS here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.