Early diagnosis of human immunodeficiency virus-1 infection in infants with the NucliSens EasyQ assay on dried blood spots

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
R R LilianG G Sherman

Abstract

More routine laboratories in South Africa are equipped to perform quantitative than qualitative HIV viral detection assays. The accessibility of early infant diagnosis would be improved if a quantitative viral load (VL) assay performed on dried blood spots (DBS) could accurately diagnose HIV-infection. The VL assay routinely used in the country has not previously been assessed on DBS for early infant diagnosis. To determine the accuracy of the NucliSens EasyQ assay (bioMerieux, Lyon, France) on DBS for early infant diagnosis of HIV in a subtype C-infected population. Stored DBS samples collected from children presenting for HIV testing were analyzed. DBS EasyQ VL results were compared to the child's HIV status as determined by a whole blood HIV DNA PCR result. The EasyQ assay was performed on 235 stored DBS samples from 71 HIV-infected and 164 HIV-uninfected children. Of the 216 infants (children aged 12 months or less) tested, all 52 HIV-infected infants were detected (sensitivity of 100%). Six of 164 HIV-uninfected infants yielded false positive results (specificity 96.3%). All false positive and six of the true positive infants had VL<3.7 log IU/ml. These 12 (5.6%) infants would require repeat testing to differentiate true f...Continue Reading

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Citations

Jun 28, 2011·Analytical and Bioanalytical Chemistry·Julien DéglonChristian Staub
Sep 13, 2012·Sensors·Chia-Chen ChangChii-Wann Lin
Dec 25, 2013·The American Journal of Tropical Medicine and Hygiene·Pieter W SmitPaul N Newton
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Sep 25, 2010·Journal of Pharmaceutical and Biomedical Analysis·Julien DéglonChristian Staub
Sep 25, 2010·Journal of Virological Methods·Marco Rossi de GasperisFrancesco Callea
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Mar 25, 2019·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Ahmad Haeri MazanderaniUNKNOWN LEOPARD Study Team

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