PMID: 11899063Mar 20, 2002Paper

Early discovery drug screening using mass spectrometry

Current Topics in Medicinal Chemistry
Marshall M Siegel

Abstract

Electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) mass spectrometric methods useful for early discovery drug screening are reviewed. All methods described involve studies of non-covalent complexes between biopolymer receptors and small molecule ligands formed in the condensed phase. The complexes can be sprayed intact directly into the gas phase by ESI-MS using gentle experimental conditions. Gas phase screening applications are illustrated for drug ligand candidates non-covalently interacting with peptides, proteins, RNA, and DNA. In the condensed phase, the complexes can be also isolated, denatured and analyzed by ESI-MS to identify the small molecule ligands. Condensed phase drug screening examples are illustrated for the ESI-MS ancillary techniques of affinity chromatography, ultrafiltration, ultracentrifugation, gel permeation chromatography (GPC), reverse phase-high performance liquid chromatography (RP-HPLC) and capillary electrophoretic methods. Solid phase drug screening using MALDI-MS is illustrated for small molecule ligands bound to MALDI affinity probe tips and to beads. Since ESI and MALDI principally produce molecular ions, high throughput screening is achieved by analyzing mas...Continue Reading

Citations

May 18, 2004·Journal of the American Society for Mass Spectrometry·Karen B GoodingRandall K Julian
Dec 17, 2004·Nature·Brent R Stockwell
Aug 12, 2010·Journal of Nucleic Acids·Frédéric RosuEdwin De Pauw
Feb 12, 2008·Biochimie·Frédéric RosuValérie Gabelica
Oct 1, 2005·Drug Discovery Today. Technologies·Matthew J ToddMarina I Nelen
Jan 13, 2006·Methods in Enzymology·Noelle PotierAlain Van Dorsselaer
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Nov 25, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Shufeng Zhou

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