Early effects of high concentrations of progesterone and mifepristone A gene expression study of endometrial cancer cells (Ishikawa)

The Journal of Steroid Biochemistry and Molecular Biology
Anne ØrboRuth H Paulssen

Abstract

Patients with endometrial hyperplasia representing preliminary stages of endometrial cancer have shown to respond to therapy in 100% of the cases when treated with levonorgestrel-impregnated intrauterine device. Anti-proliferative effect has also been reported after application of an anti-progestin impregnated intrauterine device which showed to induce endometrial atrophy. The intention of the present study was to obtain more information of novel therapeutic targets for hormonal treatment in endometrial hyperplasia and endometrial cancers. Gene expression of signaling pathways after stimulation of Ishikawa cells with high doses of progesterone (32 microM) or Mifepristone (32 microM) was performed. After using an oligo microarrays representing 24,650 human genes and 37,580 gene transcripts, 6154 genes remained after pre-processing and filtering. This resulted in a total of 993 up-regulated genes with 189 genes for progesterone and 255 genes for Mifepristone. The 550 down-regulated genes were distributed with 256 genes for progesterone, 127 genes for RU 486. The results showed that genes presenting the epidermal growth factor (EGF)/MAP-kinase pathway were significantly over-represented by progesterone treatment, whereas, by Mifep...Continue Reading

References

Jan 1, 1989·American Journal of Obstetrics and Gynecology·A Ferenczy, M Gelfand
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·Y BerthoisB S Katzenellenbogen
Sep 30, 1993·Biochemical and Biophysical Research Communications·E A Musgrove, R L Sutherland
Oct 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·O HeikinheimoG D Hodgen
Jan 1, 1997·Annual Review of Medicine·F CadepondE E Baulieu
Nov 24, 1998·Journal of the Society for Gynecologic Investigation·C C SchneiderC Gerçel-Taylor
Apr 21, 1999·Current Opinion in Cell Biology·T P Garrington, G L Johnson
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
Jun 8, 2001·Bioinformatics·O TroyanskayaR B Altman
Aug 11, 2001·The Journal of Biological Chemistry·O KovalskyA J Fornace
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 8, 2003·The Journal of Clinical Endocrinology and Metabolism·Shouwei Han, Neil Sidell
Apr 22, 2003·Human Cell·Masato Nishida
Apr 16, 2005·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Toru FurukawaAkira Horii
May 3, 2006·Molecular Carcinogenesis·Yi Sun
Oct 24, 2006·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·M NylaendeA J Kroese
Apr 24, 2007·Nature Cell Biology·Christopher J SarkisianLewis A Chodosh
Sep 27, 2007·Gynecologic Oncology·Narayan ShivapurkarSophia S Wang

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Citations

Mar 23, 2011·The European Journal of Contraception & Reproductive Health Care : the Official Journal of the European Society of Contraception·Lena MarionsInger Øverlie
Dec 20, 2011·Biopolymers·Wolfgang BrandtLudger Wessjohann
Nov 20, 2014·Human Reproduction Update·Bansari PatelSam Mesiano
May 15, 2015·Reproductive Biology and Endocrinology : RB&E·Anna BylanderD G Joakim Larsson
May 5, 2020·Endocrine Reviews·Md Soriful IslamJames Segars

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