Early embryonic death in mice lacking the beta-catenin-binding protein Duplin

Molecular and Cellular Biology
Masaaki NishiyamaKeiichi I Nakayama

Abstract

The Wnt signaling pathway plays a pivotal role in vertebrate early development and morphogenesis. Duplin (axis duplication inhibitor) interacts with beta-catenin and prevents its binding to Tcf, thereby inhibiting downstream Wnt signaling. Here we show that Duplin is expressed predominantly from early- to mid-stage mouse embryogenesis, and we describe the generation of mice deficient in Duplin. Duplin(-/-) embryos manifest growth retardation from embryonic day 5.5 (E5.5) and developmental arrest accompanied by massive apoptosis at E7.5. The mutant embryos develop into an egg cylinder but do not form a primitive streak or mesoderm. Expression of beta-catenin target genes, including those for T (brachyury), Axin2, and cyclin D1, was not increased in Duplin(-/-) embryos, suggesting that the developmental defect is not simply attributable to upregulation of Wnt signaling caused by the lack of this inhibitor. These results suggest that Duplin plays an indispensable role, likely by a mechanism independent of inhibition of Wnt signaling, in mouse embryonic growth and differentiation at an early developmental stage.

References

Jan 1, 1993·Methods in Enzymology·D G Wilkinson, M A Nieto
Feb 7, 1998·Genes & Development·K M Cadigan, R Nusse
Sep 4, 1998·Science·T C HeK W Kinzler
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·A BauerR Kemler
Jan 19, 1999·Annual Review of Cell and Developmental Biology·A Wodarz, R Nusse
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·B MannC Hanski
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·S HatakeyamaK Nakayama
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·M ShtutmanA Ben-Ze'ev
Aug 4, 1999·Nature Genetics·P LiuA Bradley
Jan 5, 2000·Cellular Signalling·A Kikuchi
Jan 5, 2000·Genes & Development·T P YamaguchiA P McMahon
Feb 5, 2000·Biochimica Et Biophysica Acta·M J Seidensticker, J Behrens
Feb 9, 2000·The Journal of Cell Biology·J HuelskenW Birchmeier
Mar 8, 2000·Mechanisms of Development·S J ArnoldR Kemler
Apr 18, 2000·The Journal of Cell Biology·K I Takemaru, R T Moon
Aug 2, 2000·The Journal of Biological Chemistry·I SakamotoA Kikuchi
Nov 1, 2000·Cell·M Bienz, H Clevers
Sep 13, 2001·Current Biology : CB·T P Yamaguchi
Sep 21, 2001·Molecular and Cellular Biology·E SadotA Ben-Ze'ev
Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·D YanL T Williams
Jan 26, 2002·Molecular and Cellular Biology·Barbara LustigJürgen Behrens
May 15, 2002·The EMBO Journal·Adam Hurlstone, Hans Clevers

❮ Previous
Next ❯

Citations

Jan 20, 2009·Nature Cell Biology·Masaaki NishiyamaKeiichi I Nakayama
Nov 16, 2011·Molecular and Cellular Biology·Masaaki NishiyamaKeiichi I Nakayama
Aug 27, 2010·American Journal of Physiology. Cell Physiology·Jennifer M RodenbergB Paul Herring
Dec 3, 2010·The Journal of Clinical Investigation·Ichiro OnoyamaKeiichi I Nakayama
Jan 7, 2016·Frontiers in Neuroscience·Rebecca A BarnardBrian J O'Roak
Jul 19, 2015·Human Molecular Genetics·Silvia De Rubeis, Joseph D Buxbaum
Jul 15, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Hung-Fu LiaoShau-Ping Lin
Jan 9, 2015·Stem Cells and Development·Joseph A MicucciDonna M Martin
Jun 6, 2009·Cell Division·Zakir UllahMelvin L Depamphilis
Jul 29, 2004·DNA Repair·Claudia C S Chini, Junjie Chen
Sep 8, 2016·Nature·Yuta KatayamaKeiichi I Nakayama
May 29, 2007·DNA Repair·Renee D Paulsen, Karlene A Cimprich
Feb 15, 2005·Experimental Cell Research·Chui Chui HoRandy Y C Poon
Aug 24, 2007·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J Adam Hall, Philippe T Georgel
Apr 17, 2007·Journal of Molecular Biology·John F FlanaganSepideh Khorasanizadeh
Apr 14, 2017·Cell Reports·Randall J PlattFeng Zhang
Feb 14, 2018·Nucleic Acids Research·Raquel FueyoMarian A Martínez-Balbás
Feb 3, 2006·Journal of Biochemistry·Keitaro YamashinaAkira Kikuchi
Mar 7, 2020·Human Molecular Genetics·Atsuki KawamuraKeiichi I Nakayama
May 28, 2020·Glia·Carlos ParrasQing Richard Lu
Jun 1, 2012·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yasuyuki KitaKeiichi I Nakayama
Feb 3, 2016·American Journal of Medical Genetics. Part a·Marta SmykBeata Nowakowska
Mar 22, 2016·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Akihiro NitaKeiichi I Nakayama
Jul 4, 2017·Nature Neuroscience·Andrea L GompersAlex S Nord
Aug 26, 2020·Proceedings of the National Academy of Sciences of the United States of America·Sabina SoodGerald R Crabtree
Feb 24, 2011·European Journal of Haematology·Alexandra FilipovichKarl-Anton Kreuzer
Jul 7, 2017·Current Developmental Disorders Reports·Jess NithianantharajahElisa L Hill-Yardin
Jan 23, 2021·Journal of Clinical Medicine·Anke Hoffmann, Dietmar Spengler
Jan 14, 2021·International Journal of Molecular Sciences·Marc-Michel WilsonGary P Brennan
Dec 3, 2017·Molecular and Cellular Neurosciences·Amanda Moccia, Donna M Martin
May 3, 2021·BMC Neuroscience·Stanislav M CherepanovHaruhiro Higashida

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis