Early events in islets and pancreatic lymph nodes in autoimmune diabetes

Journal of Autoimmunity
Caroline AspordCharles Thivolet

Abstract

The specific contributions of islet cell microenvironment during the development of autoimmune type 1 diabetes remain unclear. The aims of this study were to identify early immune-driven abnormalities in islets and pancreatic lymph nodes of NOD mice by cDNA arrays. We compared gene expression profiles of purified islets and pancreatic lymph nodes of 4-week-old NOD mice to NOD-SCID and BALB/c mice. To further characterize the networks implicated in beta-cell destruction, we also performed a time-course analysis using islets and pancreatic lymph nodes of NOD mice from 2 to 25 weeks of age. We found consistent changes by cDNA arrays and RT-PCR analyses among islet genes before the detection of CD3+ T cells in the islet periphery associated with dendritic cell attraction, lymphocyte homing, and apoptosis. In contrast to IL-1, TYNFSF13B and osteopontin genes which were specifically activated, the immunoregulatory cytokine IL-11 was poorly detected in NOD islets and pancreatic lymph nodes. Genes involved in angiogenesis were also specifically activated in NOD islets of 2 and 4 weeks of age. The present time-course macroarray and RT-PCR analyses provides a detailed picture of the different genes involved in autoimmune diabetes and ill...Continue Reading

References

Nov 1, 1996·The Journal of Experimental Medicine·P D RennertP S Hochman
Dec 10, 1999·The Journal of Experimental Medicine·F MackayJ L Browning
Jan 1, 2000·Diabetes·J D TrudeauD T Finegood
Feb 8, 2000·Stem Cells·P L Jensen
Feb 8, 2000·The International Journal of Biochemistry & Cell Biology·M Nakamoto
Feb 15, 2001·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·M BozzaW L Trepicchio
Mar 29, 2001·Diabetes·T Mandrup-Poulsen
Apr 3, 2001·Trends in Cardiovascular Medicine·R H Adams, R Klein
Jun 8, 2001·The Journal of Experimental Medicine·R EttingerH O McDevitt
Oct 26, 2001·Current Opinion in Immunology·P Santamaria
Dec 14, 2001·Nature·D MathisC Benoist
Feb 28, 2002·Trends in Immunology·Fabienne Mackay, Charles R Mackay
Apr 27, 2002·Nature·Diane Gershon

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Citations

Nov 2, 2013·Archives of Pharmacal Research·Kitae Ryu, Tae-Il Kim
Nov 9, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Katherine S BlevinsSung Wan Kim
Jan 14, 2009·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Renato MarcianoRenata Lorini
Mar 22, 2007·Clinical and Experimental Immunology·E AboumradC Thivolet
Oct 18, 2011·European Journal of Immunology·Yannick SimoniAgnès Lehuen
Aug 23, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Wan Seok JooSung Wan Kim
Apr 28, 2011·Brain, Behavior, and Immunity·Laila Y Al-ayadhi, Gehan A Mostafa
Sep 30, 2016·Clinical Reviews in Allergy & Immunology·Ayelet KaminitzNadir Askenasy
Jul 27, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jeffrey D CarterZandong Yang
Jun 27, 2017·Expert Review of Proteomics·Inne CrèvecoeurLut Overbergh
Oct 6, 2010·Multiple Sclerosis : Clinical and Laboratory Research·Lars BörnsenFinn Sellebjerg
Nov 20, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gopal MurugaiyanHoward L Weiner

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