Early events in the action of staphylococcal alpha-toxin on the plasma membrane of adrenocortical Y1 tumor cells.

Infection and Immunity
L Blomqvist, M Thelestam

Abstract

The early events in staphylococcal alpha-toxin action on mouse adrenocortical (Y1) tumor cells were studied. Cell-bound toxin could be partially neutralized by anti-alpha-toxin and inactivated by trypsin added within 10 min at 37 degrees C after the end of the binding step. Likewise, cell-bound toxin was capable of lysing rabbit erythrocytes (RRBC) added to the cells within 10 min after binding at 37 degrees C. After this time, the Y1 cells could not be rescued from intoxication by antibodies or trypsin, and the toxin was not accessible for lysis of RRBC. However, at 0 to 4 degrees C, the cell-bound toxin remained accessible to antibodies for at least 4 h. CaCl2 (30 mM) did not affect binding of the toxin to Y1 cells but completely prevented the intoxication if added within 10 min at 37 degrees C after the end of the binding step. The intoxication was independent of metabolic energy, active receptor clustering on the cell surface, and endocytosis of the toxin. Therefore, alpha-toxin interacted with the Y1 cell membrane in at least three separable steps: binding, a conformational change at the cell surface, and membrane damage. These early events appear to be similar to those occurring on RRBC treated with alpha-toxin.

References

Jun 1, 1979·Canadian Journal of Microbiology·C Y Lo, H B Fackrell
Jun 1, 1976·Biochemistry·P Cassidy, S Harshman
Jan 1, 1973·Scandinavian Journal of Immunology. Supplement·N Harboe, A Ingild
Mar 1, 1968·Journal of Bacteriology·J H FreerA W Bernheimer
Oct 1, 1981·The Journal of Cell Biology·R FüssleH J Wellensiek
Dec 19, 1983·Biochimica Et Biophysica Acta·I Florin, M Thelestam
Jan 1, 1982·Pharmacology & Therapeutics·J H Freer, J P Arbuthnott
Jan 1, 1983·Infection and Immunity·M P McGeeS Harshman

❮ Previous
Next ❯

Citations

Nov 19, 1989·Molecular and Cellular Biochemistry·C A Pasternak
Jan 1, 1988·Toxicon : Official Journal of the International Society on Toxinology·M Thelestam, L Blomqvist
Jan 1, 1988·Toxicon : Official Journal of the International Society on Toxinology·L Blomqvist, A Sjögren
Dec 18, 1987·Biochimica Et Biophysica Acta·T E Hebert, H B Fackrell
Mar 23, 1992·Biochimica Et Biophysica Acta·H Tokunaga, T Nakae
Dec 1, 1991·Microbiological Reviews·S Bhakdi, J Tranum-Jensen
Aug 1, 1987·Infection and Immunity·L BlomqvistM Thelestam

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.