PMID: 11309808Apr 20, 2001Paper

Early functional apoptotic responses of thymocytes induced by Tri-n-butyltin

Cytometry
W GrundlerM Nüsse

Abstract

Programmed cell death, also termed apoptosis, is the main focus of interest in a variety of scientific and clinical areas. For a better understanding of the mechanisms of apoptosis, from the onset of the cellular death program to the late stages of apoptosis or apoptotic necrosis, very early functional events have to be quantified because they might be involved in temporal and causal relationships between apoptosis-related key processes. We have established a flow cytometric technique to quantify time-dependent signals simultaneously with high temporal resolution (Deltat = 1 s) in living cells. With this technique, the response of cells to apoptosis-stimulating agents can be analyzed over 15 min. For this purpose, a thermostatted sample tube holder for repeatable interruption-free injection of substances into the cell suspension was developed. Early detectable fluorescence and scatter parameters were related to intracellular free Ca2+ concentration, [Ca2+]i (Indo-1 fluorometry), membrane permeability (propidium iodide [PI] influx), and cell volume (forward scatter). A T-cell line (Jurkat) served as a model system. Apoptosis was induced by the biozid Tri-n-butyltin (TBT). Dependent on the TBT concentration (0.3-10 microM), the m...Continue Reading

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Citations

Sep 3, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Stephanie L BissonnetteJennifer J Schlezinger
Nov 24, 2004·Drug Metabolism Reviews·Klaus E Appel
Apr 12, 2017·Toxicological Sciences : an Official Journal of the Society of Toxicology·Amelia H BakerJennifer J Schlezinger
Jul 2, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Werner GrundlerHeinrich Bollwein
Jul 9, 2004·Critical Reviews in Toxicology·E DoppA V Hirner
Sep 3, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Einav Yehuda-ShnaidmanJacob Bar-Tana

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis