Early growth response-1 induction by fibroblast growth factor-1 via increase of mitogen-activated protein kinase and inhibition of protein kinase B in hippocampal neurons.

British Journal of Pharmacology
Alexander H BenzE Maronde

Abstract

The transcription factor early growth response-1 (Egr-1) and the acidic fibroblast growth factor (FGF-1) are involved in many regulatory processes, including hippocampus-associated learning and memory. However, the intracellular signalling mechanisms regulating Egr-1 in hippocampal cells are not entirely understood. We used primary mouse hippocampal neurons and the mouse hippocampal neuronal cell line HT22 to investigate how FGF-1 transiently induces Egr-1 protein. This was accomplished by a range of techniques including Western blotting, immunofluorescence, specific protein kinase inhibitors and transfectable constitutively active protein kinase constructs. Protein kinase B (PKB) and mitogen-activated protein kinase (MAPK) were both initially phosphorylated and activated by FGF-1 treatment, but when phosphorylated MAPK reached maximal activation, phosphorylated PKB was at its lowest levels, suggesting an interaction between MAPK kinase (MEK-1/2) and phosphatidyl inositol-3-kinase (PI3K) during Egr-1 induction. Interestingly, pharmacological inhibition of MEK-1/2 resulted in a robust increase in the phosphorylation of PKB, which was repressed in the presence of increasing doses of a PI3K inhibitor. FGF-1-mediated Egr-1 inductio...Continue Reading

References

Jan 1, 1992·The American Journal of Clinical Nutrition·Y OomuraN Yanaihara
Aug 1, 1997·The Journal of Biological Chemistry·J D English, J D Sweatt
Feb 20, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H WichtH W Korf
Jul 1, 1996·Learning & Memory·E D RobersonJ D Sweatt
Jul 8, 2003·Cell and Tissue Research·Bernhard Reuss, Oliver von Bohlen und Halbach
May 3, 2005·Cytokine & Growth Factor Reviews·V P EswarakumarJ Schlessinger
Jun 7, 2005·Nature Neuroscience·Xi ChenDaniel R Storm
Dec 13, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jacek JaworskiMorgan Sheng
Jul 6, 2006·The European Journal of Neuroscience·Jennifer M HorwoodSabrina Davis
Feb 14, 2007·Journal of Circadian Rhythms·Sevag A KaladchibachiArmen S Manoukian
Aug 7, 2007·The Journal of Biological Chemistry·J Simon WiegertHilmar Bading
Aug 12, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hideko HayashiEisuke Nishida
Sep 26, 2008·Journal of Medicinal Chemistry·Konstantina VougogiannopoulouLaurent Meijer
Oct 28, 2008·Nature Neuroscience·Marc FlajoletPaul Greengard
Jan 23, 2009·Nature Neuroscience·Kristin L Eckel-MahanDaniel R Storm
Sep 17, 2009·Apoptosis : an International Journal on Programmed Cell Death·S Steiger-Barraissoul, A Rami
Nov 4, 2009·British Journal of Pharmacology

❮ Previous
Next ❯

Citations

Dec 19, 2013·PloS One·Alexander H BenzFaramarz Dehghani
Mar 19, 2014·Molecular and Cellular Endocrinology·Gerald ThielOliver G Rössler
Dec 17, 2014·Molecular and Cellular Neurosciences·A BrobeilM Wimmer
Jul 12, 2012·The European Journal of Neuroscience·David YangAmelia Russo-Neustadt
Feb 13, 2013·Journal of Neurochemistry·Julie BrossaudJean-Benoît Corcuff
Jul 8, 2016·Cell Proliferation·Qian JingJingyi Li
May 28, 2019·Journal of Cellular Physiology·Wenqian YangZhigang Cheng
Aug 22, 2015·Molecular Cancer Therapeutics·Yeonui KwakTaebo Sim
Oct 16, 2020·Journal of Stroke·Shuai ZhangXiaofeng Jia

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.