Early histopathology of small intestinal discordant xenografts

Transplantation
E N YedidagF P Stuart

Abstract

A descriptive study of a new model enabling serial biopsies of ongoing hyperacute rejection of small intestinal discordant xenografts is presented. In a series of guinea-pig-to-Lewis rat small bowel xenotransplants (n=7), aboral free ends of Thierry-Vella loops constructed from the graft were sequentially biopsied at one-minute intervals up to ten minutes post-reperfusion and less frequently thereafter. In a guinea pig-to-guinea pig (n=6) isograft series, biopsy controls for preservation/ischemia-reperfusion injury were obtained. Xenoantibody sequestration in this model was evaluated in a separate series of transplants, utilizing an ELISA assay for rat anti-guinea pig natural antibodies. Pathologic evaluation revealed a unique series of events characterized with microcirculatory failure and thrombosis progressing from the submucosal vasculature to the lumen. Within the system's detection limits, complement deposition and P-selectin expression occurred as early as one minute post-reperfusion, preceding the staining for IgM and IgG. Using rat serum ELISAs, no significant difference in xenoantibody sequestration was detected between the xenograft and isograft groups. The guinea pig-to-rat discordant small bowel xenotransplantation...Continue Reading

References

Dec 1, 1988·Transplantation·S MiyagawaH Kitamura
Oct 1, 1994·Immunological Reviews·F H BachW W Hancock

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Citations

Aug 29, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Leticia FernándezCarmen Peralta
Nov 4, 2000·Expert Opinion on Investigational Drugs·Z ZhangR Zhong
Jan 1, 2011·Xenotransplantation·Flávio Henrique Ferreira GalvãoLuiz Augusto Carneiro D'Albuquerque

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